ABSTRACT
Objective To investigate the changes of serum 8-hydroxy-2'-deoxyguanosine(8-OHDG) in Kawasaki disease (KD) children and to explore the importance of 8-OHDG in predicting the severity of coronary artery lesions in Kawasaki Disease.Methods The serum 8-OHDG was measured in KD patients group (n =60),fever patients group (n =12) and health control group (n =12) by ELISA method.Among the KD patients,30 KD patients were in acute stage (10 cases had coronary artery lesions,20 coronary were normal) and 30 patients were in recovery stage.The serum 8-OHDG and brain natriuretic peptide (BNP) were also compared between patients with coronary artery lesions (CALs) and patients with no coronary artery lesions NCALs).ROC curve analysis was used to test the 8-OHDG and BNP predictive values in KD with coronary artery lesions.Results The serum level of 8-OHDG was higher in acute KD patients than recovery KD patients,fever patients and healthy children (P ＜ 0.05) The serum 8-OHDG was higer in CALs patients than the NCALs patients (P ＜ 0.05).The serum BNP was higer in CALs patients than the NCALs patients (P ＜ 0.01).Analysis of the ROC curve showed that serum 8-OHDG had a positive predictive value of 64.3％ and a negative predictive value of 93.8％for distinguishing KD with CALs from KD NCALs when cutoff value was 57.02pg/ml.The area under the curve was 0.820.The serum BNP had a a positive predictive value of 47.6％ and a negative predictive value of 100％ for distinguishing KD with CALs from KD NCALs when cutoff value was 815pg/ml.The area under the curve was 0.745.Conclusion The serum 8-OHDG may have better predictive value than BNP in diagnose KD children with coronary artery lesions.
ABSTRACT
AIM: To establish an HPLC-MS method for simultaneously determining four effective components in Guilin Watermelon Frost(Mirabilitum Praeparatum,Rhizoma Coptidis,Radix Scutellariae,etc.). METHODS: The sample was extracted with methanol containing 20% chloroform under ultrasonication.The HPLC separation was performed on Zorbax SB C_(18)(3.0 mm?250 mm,5 ?m) column using water including 0.5% formic acid(A)-methanol(B) as mobile phase,with the gradient elution(0-2 min,60%B;2-5 min,60%→90%B;5 min to the end,90%B), at a flow rate of 0.40 mL/min.The compounds were analyzed by ESI-MS under ion monitoring mode(0-3 min,m/z 249;3-6 min,m/z 336;6-10 min,m/z 447;10-16 min,m/z 263). RESULTS: The linear ranges were 0.020-10.0 ?g/mL,0.010-40.0 ?g/mL,0.036-50.0 ?g/mL and 0.040-4.00 ?g/mL for matrine,berberine,baicalin and indirubin,with detection limits of 0.005,0.001,0.006 and 0.010 ?g/mL,respectively.The average recoveries ranged from 96% to 101% with all relative standard deviations less than 3%.CONCLUSION: (The method is rapid,accurate and suitable for the quality control of the four effective) components in Guilin Watermelon Frost.