ABSTRACT
Objective@#To compare the clinical effect of innervated reverse digital artery island flap and the digital artery island flap with dorsal branches in the repair of fingertip defects.@*Methods@#From January 2014 to June 2015, a total of 43 cases (50 fingers) with fingertip defects in 2-5 fingers were randomly treated with reverse digital artery island flaps (23 cases) or the digital artery island flaps with dorsal branches (20 cases). All cases underwent emergency surgery. The operative time, patient satisfaction of appearance for the reconstructed fingers, the static 2-point discrimination of the flaps, active motion of the injured finger joints and postoperative appearance on donor site were compared respectively.@*Results@#All flaps survived primarily. The follow-up period ranged from 12-18 months. No significant differences were found in operative time and active motion of the injured finger joints between the two groups (P>0.05). Patient satisfaction of appearance and the static 2-point discrimination of the flaps in reverse digital artery island flap group were better than those in the digital artery dorsal branch island flap group (P<0.05). For the donor site, the texture of donor site in reverse digital artery island flap was soft without significant Scar contracture and pigmentation. There were 5 cases with mild scar contracture in donor site at the digital artery island flap with dorsal branches.@*Conclusions@#The reverse digital artery island flap and the digital artery island flap with dorsal branches are all suitable for the repair of fingertip defects. Compared with the digital artery island flap with dorsal branch, the reverse digital artery island flap can provide better fingertip sense and appearance.
ABSTRACT
BACKGROUND: Previous studies have demonstrated that the Chinese porous tantalum made in China has non-toxicity and good biocompatibility, which can promote osteogenesis. OBJECTIVE: To investigate the effects of transforming growth factor β1 on proliferation, cel cycle and secretion of osteoblasts on porous tantalum/MG63 osteoblast-like cel composites. METHODS: Passage 3 MG63 osteoblast-like cel suspension (1×109/L) was seeded onto the porous tantalum, then the cel composites were inoculated in the medium with 0, 0.5, 5 and 10 μg/L transforming growth factor β1, respectively. The proliferation of osteoblasts was detected by cel counting kit-8 assay at 1-13 days after inoculation; the cel morphology and ultrastructure observed by scanning electron microscope and transmission electron microscopy; and level of col agen type I detected by enzyme-linked immunosorbent assay. RESULTS AND CONCLUSON: 0.5, 5, 10 μg/L transforming growth factor β1 could promote the osteoblast proliferation, and cel proliferation in the 5 μg/L transforming growth factor β1 group was higher than that in the other groups; in the 5 μg/L transforming growth factor β1 group, laminated osteoblasts adhered on the surface and grew into inner of porous tantalum, which extended more pseudopodia toward the scaffold; osteoblasts-secreted matrix could cover the scaffold and numerous rough endoplasmic reticulum, free ribosomes, dense mitochondria, Golgi apparatus as wel as matrix vesicles could be found in the cytoplasm. In addition, the level of col agen type I in the 5 μg/L transforming growth factor β1 group was significantly higher than that in the other groups (P < 0.05). These results indicate that transforming growth factor β1 can promote proliferation, and col agen type I secretion of osteoblasts on porous tantalum/MG63 osteoblast-like cel composites, and the optimum mass concentration of transforming growth factor β1 is 5 μg/L.