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Objective:To investigate the relationship between serum ferritin (SF) and metabolic associated fatty liver disease (MAFLD).Methods:A total of 7 980 adults from physical examination in Tianjin Medical University General Hospital who met selection criterion were recruited as participants. Subjects were divided into 4 groups ( Q1- Q4) according to quartiles of SF level by gender. The incidence of MAFLD in each group in 2018-2020 were observed and cumulative incidence of MAFLD was compared between each group. Cox regression model was used to analyze the correlation between different SF levels and new-onset MAFLD, and stratified analysis was performed according to factors as gender, age, smoking status and body mass index (BMI). Results:The mean follow up period was 3.2 years, and there were 25 323 person-years of follow-up, and 1 696 subjects developed MAFLD. The incidence density of MAFLD in Q1- Q4 groups were 53.30, 56.39, 72.80 and 85.78/1 000 person-years, respectively. The incidence density of MAFLD increased along with the increase of SF levels, and the differences were statistically significant ( P values between Q3, Q4 and Q1 groups were both<0.001) with SF ≥ 162.47 μg/L (male) and 49.94 μg/L (female). The risk of MAFLD were 1.173 (95% CI: 1.093-1.258) and 1.174 (95% CI: 1.122-1.228) times higher in Q3 and Q4 group than that in Q1 group ( P<0.001). After adjusting for age, smoking status and other confounding factors, the risks of MAFLD in the Q3 and Q4 groups were 1.092 (95% CI: 1.017-1.172) and 1.084 (95% CI: 1.035-1.136) times higher in Q3 and Q4 group than that in Q1 group (all P<0.001). The stratified analyses based on gender, age, smoking status, BMI and other factors indicated that high SF levels showed significant association with MAFLD risk in those with the male sex, younger age, non-smokers and higher BMI population. Conclusions:High SF levels might increase the risk of MAFLD and this positive association might be associated with gender, age, smoking status and BMI.
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Objective To explore the correlation of serum thyroid hormones with glucose and lipid metabolism in children with type 1 diabetes (T1DM) whose thyroid functions with normal.Methods 50 T1DM and 71 healthy children were enrolled in our study.Their blood glucose,lipid,HbA1c and thyroid functions were determined.Results Compared with the control group,patients with T1DM had higher low density lipoproteincholesterol (LDL-C),triglyceride(TG),fasting blood glucose (FBG) and HbA1c while with lower high density lipoprotein-cholesterol (HDL-C),thyroid Stimulating Hormone (TSH),and free triiodothyronine (FT3) (P＜0.05).In children with T1DM,TSH was positively correlated with LDL-C (r=0.175,P=0.034),total cholesterol (TC)(r=0.187,P=0.009) and TG(r=0.206,P=0.005) while negatively correlated with FBG(r=-0.187,P=O.018) and HbA1c (r=-0.264,P＜0.001).FT3 was negatively correlated with TG (r=-0.185,P=O.012),FBG (r=-0.193,P=O.O07) and HbA1c 0=-0215,P=0.002).But there were no correlations of FT4 with glucose or lipid metabolism.Conclusion TSH and FT3 are significantly correlated with glucose and lipid metabolism in children with T1DM.
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Purpose To investigate the expression of cell cycle related protein including Cyclin D1,CDK4,p16 and IgH/CCND1 fusion gene in mantle cell lymphoma (MCL) and their relationship with each other.Methods The expression of cell cycle related protein including Cyclin D1,CDK4,p16 and IgH/CCND1 fusion gene were detected on the 40 cases of MCL (expreimental group) and 20 cases of reactive hyperplasia (control group) by using the combined detection of fluorescence in situ hybridization (FISH) and immunohistochemistry of EnVision two methods.40 cases of MCL were confirmend by using gene rearrangement technique and immunohistochemistry.The threshold of IgH/CCND1 fusion gene of MCL was established in the control group.Results In the experimental group,Cyclin D1 protein positive expression rate was 100％,the positive expression of CDK4 protein rate was 87.50％,p16 protein positive expression rate was 17.50％.Positive rate of IgH/CCND1 fusion gene of 100％.These cell cycle related protein and IgH/CCND1 fusion gene were negative in the control group.Conclusion In MCL,Cyclin D1-CDK4-p16 pathway is consistent with the principle of tumor cell cycle regulation.The establishment of threshold value of IgH/CCND1 fusion gene by FISH technique may provide the basis for the judgement of FISH of the IgH/CCND1 in China.
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Purpose To investigate the corre1ation of the epiderma1 growth factor receptor( EGFR)gene mutation and amp1ification and protein expression with occurrence and deve1opment of non-sma11 ce11 1ung cancer( NSCLC),and to exp1ore the re1ationship be-tween the mutation and amp1ification of EGFR gene and other c1inica1 patho1ogica1 parameters. Methods qRT-PCR,FISH and immu-nohistochemistry were used to detect EGFR gene(exons 18,19,20 and 21)mutation,amp1ification and protein expression in paraf-fin-embedded tissues of NSCLC. Results EGFR gene(exons 18,19,20 and 21)mutation rate was 58. 18%(32/55)in NSCLC with qRT-PCR techno1ogy,in which the occurrence rate of exon 19 de1etions and exon 21 mutation of L858R was 87. 50%(28/32). EGFR gene mutation rate was significant1y different in gender,smoking history and patho1ogica1 type(P0. 05). EGFR gene amp1ification rate was 23. 64%(13/55)and its protein expression rate was 70. 91%(39/55). Both EGFR gene mutation and amp1ification was c1ose1y corre1ated(P0. 05). Conclusion EGFR gene mutation with high pro-tein expression of NSCLC is common1y found in fema1e,no-smoking and adenocarinoma patients,who are main candidates of a tyrosine kinase inhibitor( TKI)screening. EGFR gene mutation and amp1ification is typica11y corre1ated,but their consequence is unknown, which needs to be further investigated. EGFR gene mutation and amp1ification is not consistent with protein expression,its under1ying machanism is to be determined.
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Objective To investigate the effect of continuous hemofiltration in patients with acute renal injury induced by rhabdomyolysis and summarize the nursing experience.Methods Ten patients with acute kidney injury induced by rhabdomyolysis were managed with continuous hemofiltration,corresponding nursing care performed.Results After treatment,the renal function of 7 patients resumed normal,the function of 2 were improved,and only 1 needed long-term hemodialysis.After treatment,the levels of serum creatine phosphokinase(CPK),myoglobin(Mb),blood urea nitrogen(BUN),creatinine(Scr),aspartate aminotransferase(AST) were gradually decreased,K+resumed normal and urine volume increased to normal level,with significant difference as compared to those before treatment(P<0.05).Conclusions Continuous hemofiltration together with pertinent nursing measures can ensure the curative effect.It is of great significance for improving the prognosis of rhabdomyolysis.
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Objective To summarize the nursing strategies for the patients with anti-neutrophil cytoplasmic antibody associated with vasculitis and renal damage.Methods The nursing histories of 10 patients with anti-neutrophil cytoplasmic antibody associated with vasculitis and renal damage were retrospectively analyzed to summarize the nursing strategies.Results Nine of them were discharged after improvement. Seven of them had marked decrease in serum creatinine, 2 survived by hemodialysis at the outpatient section and 1 died of severe pulmonary infection.Conclusion Such strategies as close observation of patients, prevention of complications and mental care are important for the curative effect and nursing quality for the patients with anti-neutrophil cytoplasmic antibody associated with vasculitis and renal damage.
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Objective To evaluate the rationale of lowering the cutoff value of impaired fasting glucose(IFG)by studying the extent and severity of angiographic coronary artery disease(CAD)and the cardiovascular risk factors in subjects with different fasting plasma glucose(FPG)levels. Methods A total of 911 consecutive patients who had undergone coronary angiography were selected according to inclusion criteria. The subjects were studied in view of the extent and severity of angiographic CAD and the cardiovascular risk factors with different FPG levels. Results (1) Compared with the group of FPG<5.6mmol/L, the numbers of diseased vessels in the group with FPG 5.6-6.0mmol/L were significantly increased(P<0.05)after adjustment of age, sex and other influencing factors; the group with FPG 6.1-6.9mmol/L had both raised number of diseased vessels and the CAD Gensini cumulative index(P<0.01). (2) The prevalences of overweight, hypertension, hypertriglyceridemia, metabolic syndrome were progressively increasing with graded FPG levels. The prevalence of hypertriglyceridemia was significantly increased with FPG level at 5.0-5.6mmol/L(P<0.05),and the prevalences of metabolic syndrome and other components were significantly elevated with FPG level at 5.6-6.0mmol/L(P<0.05). Conclusion (1) The extent and severity of angiographic CAD were increased with increased FPG even in prediabetic period. The risk of angiographic CAD became increased significantly with FPG at 5.6-6.0mmol/L level. (2) The cardiovascular disease(CVD)risk was also increased with increasing FPG even in prediabetic period. The phenomenon of clustering of CVD risk factors was found at FPG 5.6mmol/L.
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Objective To observe the all-cause and cardiocerebrovascular disease(CCVD)-related mortalities in the senile male population and the relationship of them with fasting plasma glucose (FPG) level.Methods A survey was performed among 1 572 male subjects aged 60-90.All the subjects were groups were calculated by Kaplan-Meier method and the log-rank test was used to compare the survival rates of the 4 groups.Logistic regression model was used to analyze the correlation factors of all-cause mortality and CCVD-related regression model analysis showed that age was related with the all-cause mortality.Among all these factors, age, body mass index, FPG and the history of CCVD had the relationships with the mortality of CCVD.Conclusion In the older male population, CCVD are considered as one of the main causes of death.With the increased levels of FPG, the all-cause mortality and CCVD mortality of senile male population are increased.