ABSTRACT
OBJECTIVE：To stu dy the regulation effects of Huanglian jiedu decoction on M 1 and M 2 macrophage polarization in atherosclerosis （AS）model mice ，and to elucidate its mechanism of AS prevention and treatment. METHODS ：Sixty ApoE －/－ male mice were randomly divided into blank control group ，model group ，simvastatin group [positive control ，5 mg/（kg·d）]， Huanglian jiedu decoction low-dose ，medium-dose and high-dose groups [ 5，10，20 mg/（kg·d），by crude drug] ，with 10 mice in each group. Except for blank control group ，other groups were given high-fat diet to induce AS model. After modeling ， administration groups were given relevant medicine intragastrically ；blank control group and model group were given normal saline intragastrially，once a day ，for consecutive 4 weeks. After medication ，the contents of triglyceride （TG），total cholesterol （TC）， low density lipoprotein cholesterol （LDL-C） and high density lipoprotein cholesterol （HDL-C） in serum were detected by automatic biochemical analyzer. Sirius red staining was used to observe the formation of collagen fibers in the aorta of mice. The serum contents of iNOS and CD 206 were determined by ELISA. mRNA expression levels of IL- 1β，iNOS，TNF-α，YM1 and Fizz1 in the aorta were detected by RT-PCR. RESULTS ：Compared with blank control group ，the serum contents of TC ，TG， LDL-C and iNOS ，mRNA expression levels of IL- 1β，iNOS，TNF-α in the aorta were significantly increased in model group（P＜ 0.05 or P＜0.01），while the serum contents of HDL-C and CD 206 and mRNA expression levels of IL- 10，YM1，Fizz1 in the aorta were significantly decreased （P＜0.01）. There was a thick layer of collagen fibers under the endothelium of aorta. Compared with model group ，above serum indexes of mice were improved significantly in administration groups （P＜0.05 or P＜0.01）；mRNA expression levels of IL- 1 ，iNOS and TNF-α in the aorta in Huanglian jiedu decoction medium-dose and high-dose groups were decreased significantly （P＜0.01），while mRNA expression levels of IL- 10，YM1 and Fizz 1 were increased significantly （P＜0.05 or P＜0.01）. The vascular endothelium was relatively smooth. CONCLUSIONS ：Huanglian jiedu decoction can inhibit the polarization of M 1 macrophages and promote the polarization of M 2 macrophages，reduce the inflammatory reaction ，maintain the stability of atherosclerotic plaque in artery ，so as to play the role of anti-AS.
ABSTRACT
OBJECTIVE：To systematically evaluate therape utic efficacy of Yixinshu preparation combined with conventional treatment plan for angina pectoris of coronary heart disease （CHD）vs. conventional treatment plan ，and to provide evidence-based reference for clinical drug use. METHODS ：Retrieved from Cochrane library ，PubMed，Embase，CBM，CNKI，Wanfang database and VIP ，randomized controlled trials （RCTs）about Yixinshu preparation combined with conventional treatment plan （trial group ） versus conventional treatment plan （control group ）for angina pectoris of coronary heart disease were collected during Jan. 1st in 2012 to Oct. 1st in 2020. After extracting the data ，the quality of included studies were evaluated with Cochrane System Evaluator Handbook 5.3；Meta-analysis was performed by using Stata 15.0 software. RESULTS ：A total of 23 RCTs involving 2 853 subjects were included. The results of Meta-analysis showed that the efficacy of angina pectoris [RR ＝0.92，95％CI（0.87，0.97），P＜0.05]， the times of angina pectoris attack [SMD ＝－0.98，95％CI（－1.09，－0.87），P＜0.05]，the duration of angina pectoris [SMD ＝ －0.77，95％CI（－0.88，－0.67），P＜0.05]，ECG curative effect [RR ＝0.91，95％CI（0.84，0.98），P＜0.05] and the improve rate of TCM syndromes [RR ＝0.89，95％CI（0.85，0.94），P＜0.05] in trial group were all better than control group ，with statistical significance. CONCLUSIONS ：Yixinshu preparation combined with conventional treatment plan is better than conventional treatment plan in terms of improving therapeutic efficacy of angina pectoris and ECG ，TCM syndrome improvement rate ，as well as reducing the frequency of angina pectoris attack ，angina pectoris attack duration.
ABSTRACT
OBJECTIVE： To establish the method for content determination of eugenol in Syzygium aromaticum oil dropping pills， and to optimize the preparation technology. METHODS： The content of eugenol in S. aromaticum oil dropping pills was determined by UV spectrophotometry. Based on single factor test， using the percentage of drugs in total amount， liquid temperature， falling distance of condensate， liquid drop distance as factors， taking the roundness， weight and hardness difference and comprehensive score as factors， L9（34） orthogonal design test was adopted to optimize the preparation process. RESULTS： The linear range of eugenol was 15.15-45.45 μg/mL（r＝0.999 6）； RSDs of precision， stability and reproducibility tests were all lower than 1％； the recoveries were 97.41％-100.59％（RSD＝1.35％， n＝6）. The optimal preparation technology included that the percentage of drugs in total amount was 5％； liquid temperature was 80 ℃； falling distance of condensate was 13 cm； liquid drop distance was 6 cm. The dropping pills had smooth appearance， good roundness and moderate hardness； the average content of engenol was 4.073％（RSD＝0.35％，n＝6）. CONCLUSIONS： The established method is simple， and can be used for the content determination of eugenol in S. aromaticum oil dropping pills. The optimal preparation technology is stable and feasible.