ABSTRACT
(Hp) is widely disseminated in human, and Hp infection causes various gastrointestinal diseases, including gastric cancer. Different genotypes of Hp may cause different diseases, so the genotyping is important for clinical and basic research of Hp. This article introduces the methods for Hp genotyping, including multilocus sequence typing, pulsed-field gel electrophoresis, random amplified polymorphic DNA, amplified fragment length polymorphism, and whole-genome sequencing. By reviewing the application of these techniques in Hp genotyping and comparing their advantages and disadvantages, the article provides a theoretical basis for research into the pathogenesis, antibiotic resistance, and epidemiology of Hp infection.
Subject(s)
Humans , Amplified Fragment Length Polymorphism Analysis , Genotype , Helicobacter Infections , Microbiology , Pathology , Helicobacter pylori , Genetics , Polymerase Chain Reaction , ResearchABSTRACT
ObjectiveTo investigate the molecular mechanism in the pathogenesis of mild chronic hepatitis B from the perspective of microRNA (miRNA) regulation. MethodsA total of 16 patients who visited the outpatient service of Chengdu Hospital of Infectious Diseases from July 2013 to February 2014 were enrolled and divided into mild chronic hepatitis B group and normal group. The Agilent Human miRNA 8×60 k microarray chips were used to detect the expression profile of miRNA in plasma and obtain the profile of miRNAs expressed differentially between the two groups. The miRNA bioinformatics analysis software was used to predict target genes, and GO functional enrichment analysis and pathway analysis were performed for these target genes. An analysis of variance was used for comparison between multiple groups, and the t-test was used for comparison between two groups. ResultsA total of 54 miRNAs were differentially expressed between the two groups (all P＜0.05), and among them, 30 were upregulated, and 24 were downregulated. The functions of these miRNAs included cell proliferation, positive/negative transcription regulation, positive/negative regulation of biosynthesis, protein localization, the Wnt receptor signaling pathway, positive/negative regulation of gene expression, the enzyme-linked receptor protein signaling pathway, and phosphorylation of protein amino acids. Pathway analysis revealed that miRNAs were mainly involved in the Wnt signaling pathway, Notch signal transduction pathway, Hedgehog signaling pathway, p53 signaling pathway, and B cell receptor signaling pathways, etc. ConclusionThe pathogenesis of mild chronic hepatitis B is regulated by specific miRNAs and involves various life processes and pathways.
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Objective:To evaluate the effectiveness of psychological intervention on improving the survival quality of patients with endometrial cancer during the operation. Methods:Sixty-two patients included in the eligible subjects were randomly divided into experimental group (n=31) and control group (n=31). The control group was given routine nursing intervention and the experimental group was given psychological intervention. Admission, discharge and discharge after one year follow-up measurement of quality of life were recorded. Results:Two patients discharged from the SF-36 scale score difference were statistically significant (t=11.3152, P<0.05), statistically significant long-term effect one year after the score difference (t=9.2025, P<0.05), the test group was better than control group. Conclusion:Psychological intervention is effective to improve the quality of life of patients with endometrial cancer during operation period and long-term.
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Objective To research the chronic respiratory infectious diseases caused by Pseudomonas aeruginosa need to objectively the reflection of the real environment in the body, so established a mouse model of chronic pulmonary infection with Pseudomonas aeruginosa and analyzed it's inflammatory response. Methods Establishment of chronic bronchial infection mice model that were inoculated with Pseudomonos aeruginosa-laden agarose beads, and analyzed its inflammatory response by detected the cytokines and MMP-2, and a differential cell count and a total leukocytes count were performed as well. Results Pseudomonas aeruginosa had been detected after infection, and there were changes in pathological. Pulmonary inflammation appeared in 1 d and reached near baseline levels by 7 d after inoculation. It was verified that the peak of inflammatory reaction in Pseudomonas aeruginosa infection is in 2-3 d; the mice did have detectable levels of circulating matrix metalloproteinase-2 ( MMP-2 ) after infection with Pseudomonas aeruginosa. MMP-2 concentrations in the blood serum peaked at 3 d after inoculation. It is indicated that Pseudomonas aeruginosa can initiate a certain degree of pulmonary fibrosis on the basis of the pulmonary inflammation. Conclusion In this study, chronic bronchial infection animal model affected by Pseudomonas aeruginosa was established successfully. Base on this animal model, we can do the pathogenicity and drug resistance of Pseudomonas aeruginosa further study.
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Objective To discuss the validity and the feasibility of modified specific immunotherapy of hypoglossis allergen in the treatment of children allergic asthma. Methods 100 children with allergic asthma were selected from October,2007 to April ,2008 in our hospital and divided into the observation group and the control group. The control group adopted routine method, the observation group made modifi-cation based upon routine nursing, placing emphasis on intervention of cognition and behavior of children and their parents, improvement of treatment compliance, whole- process, dynamic and continuous observa-tion of the treatment process, making individualized health education plan. The treatment compliance, total score of asthma control, and pulmonary function examination of impulse oscillation(IOS) were compared be-tween the two groups. Results The observation group was superior to the control group in treatment com-pliance, pulmonary function examination and the control results of asthma. Conclusions Specific im-munotherapy of hypoglossis allergen combined with modified nursing method can increase treatment com-pliance of children and lead to better results.
ABSTRACT
Helicobacter pylori is able to colonize the human stomach and dwell in the human stomach for decades or for whole lifetime. A number of potential virulence factors contribute to Helicobacter pylori to colonize this unusual niche. Motility is an essential colonization factor based on the fact that nonmotile variants of Helicobacter pylori can't infect gnotobiotic piglets. Motility is not a colonization factor based on rapid loss of motility of Helicobacter pylori in the gastric lumen in vivo. The exact roles of Helicobacter pylori motility are not yet known. The aim of this article is to discuss correlation between colonization and motility of Helicobacter pylori.