[ effects of vitamin E and exogenous testosterone co-administration on biochemical serum parameters of varicocele rats]

Because of impairment of testosterone production and occurrence of oxidant disorders in testis of varicocele [VCL] patients, these patients are treated with testosterone and various antioxidant. This study was performed with the objective of determining the effect of co-administration of these two drugs on the serum biochemical parameters of rats. In this study, 30 male rats were divided into 5 groups [n=6, each] of control-sham and test groups. In the control-sham group, only simple laparotomy was performed. In the test groups, experimental VCL was induced in all test groups. No treatment was performed for the first test group. The second test group was treated with testosterone [44microg/kg, ip], the third group with vitamin E [150mg/kg, orally] and the fourth group with co-administration of testosterone and vitamin E. After the end of treatment period, anesthesia and heart blood sampling were performed, and after serum separation, serum levels of glucose, creatinine, urea, total cholesterol, and triglyceride were measured. The data were analyzed using two-way variance analysis. The significance level was considered to be p < 0.05. In this study, the serum levels of the glucose, creatinine, urea, total cholesterol, and triglyceride significantly increased following VCL induction, as compared to control group [p < 0.05]. This was while single administration of testosterone and co-administration with vitamin E significantly reduced the serum levels of mentioned parameters [p < 0.05]. The results showed that co-administration of testosterone and vitamin E, in comparison with separate administration of each compound, improved the serum biochemical parameters in VCL-induced animals

[ effect of vitamin E and dexamethasone co-administration on varicocle-induced biochemical and histological alterations of liver in rats]

Varicocele [VCL] not only has functional and structural effects on the male reproductive system, but also affects other body tissues such as liver. Therefore, dexamethasone [DEX] and vitamin E [VE] co-administration is recommended invaricocele patients. The present study was conducted with the purpose of evaluating the effect of experimentally-induced varicocele on liver and the protective effects of VE and DEX. In this study, 30 male rats were divided into five groups. The experimental group were assigned to 4 groups: Varicocele; varicocele+VE [150/kg]; varicocele+DEX [0.25mg/kg]; and varicocele+VE+DEX. The control group underwent a simple laparotomy. After 60 days, the rats were anesthetized and blood was taken for serum isolation. Simultaneously with taking blood samples,the serum levels of glucose, alanine aminotransferase [ALT], and aspartate aminotransferase[AST] were evaluated. The hepatocytes' cytoplasmic carbohydrate level was assessed by periodic acid shiff staining. Statistical analysis was performed by analysis of variance and Tukey's test. The significance level was considered as p<0.05. The levels of blood glucose, ALT, and AST were significant lyreduced in VE and DEX groups compared to varicocele group. Also, tissue necrosis, central venous dilatation, and lymphatic cells infiltration decreased. According to the findings of this study, varicocelecauses liver damage at the biochemical and histological levels, and co-administration of VE and DEX could significantly reduce the VCL-induced complications and also DEX-induced side effects as well