ABSTRACT
The ability to improve the efficacy of an inactivated Newcastle disease vaccine by addition of immunomodulator as propionibacterium and lipopolysaccharide [TM-104] was studied. Chickens were divided into three groups, one received inactivated Newcastle vaccine containing the IM-104, second group received vaccine alone and third group of chickens was left without vaccination as control. Blood samples were collected 3 days post vaccination, and weekly till four weeks for estimation of the effect of [IM-104] on Tlymphocyte transformation [cellular immune response]. Sero-conversion for sera at weekly intervals was done to evaluate the immune response using HI test and ELISA. Challenge test was carried out on 3[rd] and 6[th] week post vaccination for evaluation of protective efficacy of these vaccines. The results obtained revealed that [TM-104] that contain 2 extraction of E. coli lipopolysaccharide and propioni-bacterium plays roles in stimulating both T-lymphocyte and macrophage to release different types of interleukin and consequently released immunoglobulins. Also, stimulate non-specific resistance to viral infection. The results obtained by challenge test run in parallel with that of serological test