ABSTRACT
We present a unique case of endophthalmitis with Staphylococcus lugdunensis following dexamethasone intravitreal implant for branch retinal vein occlusion associated with cystoid macular edema. Patient did not show favorable clinical response after vitrectomy and intravitreal antibiotics; so, we decided to repeat vitrectomy, remove the steroid implant and fill the eye with silicon oil, and repeat intravitreal vancomycin. Vision has improved from hand movements at presentation to counting fingers at 1.5 m after second vitrectomy and final visual acuity 3 months later after silicon oil removal was 6/36.
ABSTRACT
Chlamydia trachomatis and Listeria monocytogenes are important reproductive tract pathogens in a wide variety of animals including sheep. In humans, chronic or repeated infection of the female genital tract with hlamydia trachomatis has been identified as a significant factor in the development of occlusive infertility f increased risk of ectopic pregnancy. The aim of the current study is to detect circulating imunoglobulins in serum of sheep naturally infected with Chlamydia trachomatis and Listeria Monocytogenes in two Egyptian provinces. A total of 82 serum samples from 4 sheep flocks in two gyptian provinces were examined with enzyme-linked immunosorbent assay [ELISA] for the detection of antibodies of Chlamydia trachomatis and Listeria monocytogenes. The results of ELISA revealed the 4 detection of antibodies against the two pathogens at a very high percentage [98.78% and 100% for Chlamydia trachomatis and Lisieria monocytogenes; respectively] despite of absence of elevated abortion rates in the examined sheep flocks. It could be concluded that chlamydial and listerial infections occur frequently in Egyptian sheep flocks, even in the absence of elevated abortion rates and there is possible risk; to pregnant women in rural areas where chlamydial and listerial infections in farm animals are widespread. Also, ewes that experienced Chlamydia trachomatis or Listeria monocytogenes induced abortion provide a unique opportunity to study the host: parasite dynamic as it relates to persistent infection. This natural model of persistent infection may, in some ways, be superior to more contrived models in which the chlamydial or listerial isolate is not a normal reproductive pathogen of the study animal. Thus, the study of persistent Chlamydial or Listerial infection in sheep may be used for the benefit of both human and veterinary medicine
ABSTRACT
Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms [PCR-RFLP] and Single Nucleotide polymorphism [SNP] techniques were used to study the association between bone morphogenetic protein receptor IB [BMPR IB] gene polymorphism with litter size trait and kids growth. Forty four Female goats were precisely selected according to their litter size and kids growth. PCR amplification of 190 bp of the BMPR-IB gene was genotyped in all goats and sequenced only in those produced the highest and lowest litter size and kids growth. Restriction analysis of PCR-RFLP using Ava II and Hind III of the BMPRIB gene [190-bp] do not produce restriction fragments. By DNA sequencing, eight single nucleotide polymorphisms [SNP's] at seven different positions were obtained. Furthermore, with translation of SNPs to corresponding amino acids, change of six amino acids in three female goats were obtained as the following, Baladi goat with high litter size, glutamic acid [E] changed to aspartic acid [P] and isoleucine [I] changed to valine [V]. In high litter size, Zaraibi goat, valine [V] changed to leucine acid [L] and glutamine [Q] changed to histidine [H] and threonine [T] changed to proline [P]. These findings can be used in a marker-assisted selection [MAS] for selection for high litter size trait in goats. There are negative relationships in most goats between SNPs in BMPR IB gene and relative growth gain [RGG]