ABSTRACT
Background: Undesirable fetal outcomes and reproductive failures mayfollowToxoplasma, Cytomegalovirus [CMV] and Rubella infections
Objective: Detection of Toxoplasma, Rubella and CMV infections among aborted women attending Sohag University Hospital and to determine factors related to these infections
Methodology: Blood samples were gathered from 121 aborted women attended the hospital, from June 2016 to February 2017. Samples were examined for specific IgM and IgG antibodies against Toxoplasma, Rubella, and CMV by ChemiluminescentMicroparticle Immunoassay [CMIA] technology
Results: About two thirds [64.5%] of participants were positive for at least one of the studied infections. The specific IgM antibodies were positive in 1 case for Toxoplasma,3 cases for Rubella, and 5 cases for CMV. Specific IgG antibodies were positive in 45 cases for Toxoplasma, 33 cases for Rubella, and48 cases for CMV. Multiple logistic regression analysis identified that rural residence was significantly linked to Toxoplasma [OR=8.35, p-value <0.001], CMV [OR=2.26, p-value = 0.042], Rubella [OR = 3.7, p-value=0.003] infections. Seropositivity for multiple infections was detected in 33.1% of participants. Rural residence and high parity were significantly connected with multiple infections [p-value <0.001 and 0.045 respectively]
Conclusion: Infection with the studied agents is highly prevalent [64.5%] among the studied women where antibodies againstToxoplasma, Rubella virus and CMV were found in different proportions [38 %, 29.8 %, and 43.8 % respectively]. Rural residence is a strong predictor of these infections. Hence the country's health authorities must be alerted, and preventive measures should be taken
ABSTRACT
The present work to evaluate whether there is a significant relationship between atherosclerosis and Chlamydophila Pneumoniae [C. pneumoniae] infection by using the Enzyme Linked Immunosorbent Assay [ELISA] techniques to detect the antibodies, and polymerase chain reaction [PCR] to detect the microbial DNA. Case-control study was carried out on atheromatous plaque and serum samples obtained from 74 patients with peripheral arterial disease caused by atherosclerosis and another 14 patients with varicose veins as a control group in the Vascular Surgery Department, Sohag University Hospital over a period between October 2013 and June 2014. Chlamydophila pneumoniae DNA was extracted, amplified and detected using PCR assay, and anti C. pneumoniae IgG antibodies were detected by ELISA assay. Both ELISAandPCR techniques were done for the studied cases and the control group. ELISA technique was positive for Chlamydophila pneumoniae IgG antibody in 40 patients [54.05%] among the studied cases while in the control group, it was positive only in 4 cases [28.57%] with p-value 0.08. PCR technique was positive in 28 patients [37.84%] in the cases for Chlamydia pneumoniae 16sRNA gene, while for the control group, all cases were negative with p- value 0.005. Conclusions: Chlamydophila pneumoniae infection can be considered as a strong risk factor for atherosclerosis