ABSTRACT
OBJECTIVE: Identify the influence of ovarian hormone deprivation in expression genes on the lower urinary tract of rats. MATERIALS AND METHODS: This study deals with gene screening on lower urinary tract of rats. Fifty isogenic rats divided in two groups of twenty-five animals have their lower urinary tract surgically removed: group I, ovariectomized rats 30 days prior to surgery; group II, non-ovariectomized rats. Total RNA was isolated from bladder and urethra, and differential expression of genes was analyzed quantitative, qualitative and comparatively by array technology and RT-PCR. RESULTS: A total of 76 candidate genes were identified as differentially expressed between the groups, 26 being lower expressed in group II, and 50 in group I. Among them, differential expression validation was confirmed by RT-PCR for three lower expressed genes in group I: Vascular Endothelial Growth Factor (VEGF), Beta-2 Microglobulin (B2M) and Cytochrome c Oxidase subunit I (COX I). CONCLUSION: Ovarian hormone deprivation influences the expression genes on lower urinary tract. We demonstrated that a 30-day period of castration down regulate the expression of VEGF, B2M and COX I in adult rats which are involved in activities of angiogenesis, immune responses and cellular metabolism respectively.
Subject(s)
Animals , Female , Rats , Estrogens/deficiency , Gene Expression , Urinary Tract/metabolism , Urologic Diseases/genetics , Vascular Endothelial Growth Factor A/genetics , Cyclooxygenase 1/genetics , Disease Models, Animal , Estrogens/pharmacology , Gene Expression Profiling , In Situ Hybridization , Membrane Proteins/genetics , Oligonucleotide Array Sequence Analysis , Ovariectomy , Ovary/physiology , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Urinary Tract/drug effects , Urologic Diseases/metabolism , Vascular Endothelial Growth Factor A/blood , /geneticsABSTRACT
O Carcinoma adenóide-cístico é uma neoplasia rara na mama, representando menos de 1 por cento dos carcinomas deste orgao. Apresenta comportamento local pouco agressivo e raramente metástases, o que lhe confere um prognóstico favorável. Apresenta-se caso de carcinoma adenóide-cístico em ênfase nos critérios diagnósticos e características que indicam pior prognóstico.
Subject(s)
Humans , Female , Middle Aged , Breast Neoplasms/pathology , Carcinoma, Adenoid Cystic/pathology , Tamoxifen/therapeutic use , Breast Neoplasms/surgery , Mammography , Carcinoma, Adenoid Cystic/surgery , PrognosisABSTRACT
A biopsia aspirativa com agulha fina é uma técnica citológica para diagnóstico, necessitando de uma amostra bem menor que a necessária para o exame histológico. Com o aperfeiçoamento das técnicas de diagnóstico por imagem (ultra-sonografia, tomografia computadorizada), a biópsia aspirativa com agulha fina tem se expandido para alcançar praticamente qualquer órgäo do corpo e lesöes pequenas. Esta revisäo visa atualizar o médico da importância da biópsia aspirativa com agulha fina no auxílio ao diagnóstico, mostrando suas indicaçöes, vantagens e complicaçöes