ABSTRACT
The aim of the present study was to determine the antimicrobial and cytotoxic activity of different parts of the plant Moringa oleifera Lam. Disc diffusion method and brine shrimp lethality bioassay were used for antimicrobial activity and cytotoxic activity respectively. Chloroform fractions of leaf and fruit part have shown moderate antimicrobial activity with zone of inhibition (ZOI) ranging from 9-28 mm against all the experimental microbes. Ethyl acetate fraction of bark and fruit found to have highest antimicrobial activity with zone of inhibition (ZOI) 36 mm against Shigella dysphoria. Pet ether fraction of bark but not leaf showed activity against Bacillus megaterium. Pet ether fraction of bark showed highest activity against Candida albicans with zone of inhibition (ZOI) of 35 mm while chloroform fraction of the leaf showed highest activity against Bacillus megaterium with zone of inhibition (ZOI) of 25 mm. All the fractions were found to have potential cytotoxic activity having LC50 values ranging from 0.43-1.18 μg/ml in comparison with vincristine sulphate having LC50 value of 0.53 μg/ml. Ethyl acetate fraction of fruit showed highest cytotoxic activity with LC50 value of 0.43 μg/ml while pet ether fraction of bark showed lowest cytotoxic activity with LC50 value of 1.18 μg/ml.
ABSTRACT
The present study was designed to investigate the antioxidant and cytotoxic activity of the methanol, ethyl acetate and n-hexane extracts of bark of Aegle marmelos. The antioxidant properties was assessed by using 1, 1-diphenyl-2- picrylhydrazyl radical (DPPH) and Nitric Oxide (NO) scavenging capacity. The methanolic, ethyl acetate and n-hexane bark extracts of Aegle marmelos showed potential DPPH free radical scavenging activity having IC50 values of 37.056 μg/ml, 43.379 μg/ml and 66.180 μg/ml respectively compared with the IC50 value of Ascorbic acid 33.447 μg/ml. The Ethyl acetate extract of Aegle marmelos displayed excellent antioxidant activity in nitric oxide (NO) scavenging capacity assay with IC50 value of 28.377 μg/ml compared with the standard ascorbic acid, IC50 value of 22.175 μg/ml. The cytotoxicity assay was performed by applying brine shrimp lethality bioassay method. Among all the fractions, n-hexane extract shown profound cytotoxic activity with LC50 value of 4.482 μg/ml that was followed by methanolic and ethyl acetate having LC50 values of 5.278 and 5.278 μg/ml respectively in comparison with the standard vincristine sulfate with LC50 value of 3.364 μg/ml. The findings of the present study suggest that the extracts from Aegle marmelos bark have potential cytotoxicity and antioxidant effects.
ABSTRACT
The present study was undertaken to explore the antioxidant potential of different parts of the plant Pandanus odorus that is believed to be useful in the treatment of diabetes.1,1-diphenyl-2-picrylhydrazyl (DPPH) was used in determining the free radical scavenging activity and ascorbic acid was used as standard. All the fractions of both leaves and root showed potential antioxidant activity. In case of leaves the chloroform fraction showed highest radical scavenging activity with IC50 value of 1.66 μg/ ml followed by petroleum ether and ethyl acetate fraction having IC50 value of 4.69 μg/ ml and 26.21 μg/ ml respectively. In case of root, the petroleum ether fraction showed highest radical scavenging activity with IC50 value of 1.42μg/ ml followed by ethyl acetate and chloroform fraction having IC50 value of 3.76 μg/ ml and 7.01 μg/ ml respectively.