Toll-like Receptor 1 Polymorphisms Increased the Risk of Pulmonary Tuberculosis in an Iranian Population Sample / 生物医学与环境科学（英文）

A case-control study was carried out that involved 203 individuals diagnosed with pulmonary tuberculosis (PTB) and 203 healthy subjects. Genotyping of TLR1 rs5743551 and rs5743618 polymorphisms was done using polymerase chain reaction-restriction fragments length polymorphism assay. We found that TLR1 rs5743551 variant affected the risk of PTB in the codominant (OR=3.28, 95% CI=1.98-5.45, P<0.0001, GA vs. GG; OR=1.86, 95% CI=1.05-3.28, P=0.033, AA vs. GG) and dominant (OR=2.69, 95% CI=1.67-4.34, P<0.0001, GA+AA vs. GG) inheritance models tested. The A allele was associated with a higher risk of PTB than the G allele (OR=1.33, 95% CI=1.01-1.75, P=0.049). The TG genotype of the rs5743618 variant significantly increased the risk of PTB compared to the risk associated with the TT genotype (OR=3.29, 95% CI=1.82-5.97, P<0.0001). The G allele was associated with a higher risk of PTB than the T allele (OR=3.00, 95% CI=1.69-5.31, P=0.0001). Our findings revealed that TLR1 rs5743551 and rs5743618 polymorphisms affected the risk of PTB in an Iranian population sample. Additional studies with larger sample sizes and involving subjects of different ethnicities are required to validate our present findings.

Morphine sulphate toxicity on liver function tests in fructose-induced insulin resistant male rats

Since liver is a gland which has an important role in drug metabolism, the present study was conducted to evaluate the effect of a single dose and repeated administration of morphine on LFT, blood sugar and fasting insulin resistance index in fructose- fed male rats. The experiment was performed on 36 Wistar-Albino male rats, which were divided into a control [A] and three tests groups [B, C and D]. The control group consumed tap water, but the test groups consumed fructose-enriched water [10%, w/v] and received null, single, and repeated doses of morphine, respectively. At the end, animals were anesthetized and blood samples were collected. Liver enzymes, insulin and insulin resistance were measured. Data were analyzed by SPSS-11, using ANOVA and Tukey tests as post hoc test. Results were expressed as mean +/- SD and Statistical differences were recognized significant by p<0.05. The results showed that all test groups were insulin resistant; alanine aminotransferase [ALT] and asparatate aminotransferase [AST] activity values in group D significantly increased compared to other groups while its plasma glucose and insulin values showed a significant decrease in comparison to other test groups. It seems that repeated morphine administration can affect liver function test [LFT] and fasting Insulin resistance index [FIRI] in fructose- fed male rats

Effect of urtica dioica decoction on serum glucose and lipid profile in stereptozotocin induced diabetic male rats

Since Urtica dioica is a traditional treatment plant and is used for antihypertensive, antilipidemic and antidiabetic agents, this survey was carried out to evaluate the effect of Urtica dioica decoction on serum glucose and lipid profile in diabetic male rats induced by stereptozotocin [STZ]. This experiment was performed on 30 Wistar-Albino male rats, weighing 200-250 g, which were divided in sham control [A], diabetic control [B] and diabetic test groups [C] randomly [N=10]. Type I diabetes was induced by single intraperitoneal injection of STZ [65 mg/kg]. Test group received 0.40-0.60 ml of Urtica dioica decoction for a month by gavages, control group [B] received the same volume of distill water. Group [A] did not receive any agent during the experiment period. Finally, animals were anesthetized, sacrificed and blood samples were collected from the cervical vein. Then, serum glucose and lipid profiles were measured by ordinary methods. Data were analyzed by SPSS-11, using ANOVA and post hoc Tukey tests. Results were expressed as mean +/- SD, and statistical difference was considered significant by p<0.05. Results in the present study showed that fasting blood glucose [FBS], total cholesterol [TCho], triglyceride [TG], LDL, food and water intake were significantly decreased in group C compared with those of group B, but body weight gain was significantly increased compared with that of control group [B]. These results indicated that Urtica dioica decoction caused decreased FBS and improved serum lipids in diabetic male rats. Some more studies have shown the same mechanism