Didymin protects against polystyrene nanoplastic-induced hepatic damage in male albino rats by modulation of Nrf-2/Keap-1 pathway

Polystyrene nanoplastics (PS-NPs) are ubiquitous environmental pollutants that can cause oxidative stress in various organs, including the liver. Didymin is a dietary flavanone that displays multiple pharmacological activities. Therefore, the present study evaluated the palliative role of didymin against PS-NPs-induced hepatic damage in rats. Albino rats (n=48) were randomly distributed into 4 groups: control, PS-NPs treated group, PS-NPs + didymin co-administered group, and didymin supplemented group. After 30 days, PS-NPs intoxication lowered the expression of Nrf-2 and anti-oxidant genes [catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GSR), glutathione-S-transferase (GST), and heme oxygenase-1 (HO-1)], whereas the expression of KEAP1 kelch like ECH associated protein 1 (Keap-1) was increased. PS-NPs exposure also reduced the activities of anti-oxidants enzymes (CAT, SOD, GPx, GSR, GST, GSH, and OH-1), while malondialdehyde (MDA) and reactive oxygen species (ROS) levels were increased. The levels of alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were increased in PS-NPs-exposed rats. Moreover, inflammatory indices [interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), nuclear factor-kappa B (NF-κB), and cyclooxygenase-2 (COX-2)] were increased in PS-NPs-exposed rats. Furthermore, PS-NPs intoxication increased the expressions of apoptotic markers including Bax and Caspase-3, as well as reducing Bcl-2 expression. The histopathological analysis showed significant damage in PS-NPs-treated rats. However, didymin supplementation ameliorated all the PS-NPs-induced damage in the liver of rats. Therefore, it was concluded that didymin can act as a remedy against PS-NPs-induced liver toxicity due to its anti-apoptotic, anti-oxidant, and anti-inflammatory activities.

Profil Épidémiologique et Mycologique de l'Otomycose à l'Hôpital Général de Référence de Niamey (Niger) / Epidemiological and my cologica l profile of otomycosisat the Genera l Reference Hospital o f Niamey (Niger

But. Décrire les caractéristiques épidémiologiques et mycologiques de l'otomycose à Niamey. Méthodologie.Il s'est agi d'une étude rétrospective menée sur 3ans au service d'ORL et CCF de l'Hôpital Général de Référence, portant sur les patients chez qui le diagnostic d'otomycose a été posé. Les paramètres étudiés étaient épidémiologiques et mycologiques. Résultats. En 3 ans, nous avons enregistré 447 consultations pour otites dont 56 avaient une otomycose, soit une fréquence de 12,53% de l'ensemble des otites et 58,92% des otites externes. L'âgemoyen était de 35,75 ans (extrêmes de 2 et 70 ans). La tranche d'âge de 31 à 60 ans représentait 58,93% des patients. Nous avons retrouvéune prédominance féminine (67,85%), (p=0,0752). Les principaux facteurs favorisant l'otomycose étaient le nettoyage de l'oreille au coton tige ou à la plume de volaille : (53,57%),le port du turban ou de couvre-chef, (46,42%), la baignade (16.07%), l'étroitesse du conduit auditif externe (8,92%) et le port de prothèse auditive externe intra-conduit (5,35%,). Sur le plan mycologique, le Candida albicanset l'Aspergilus nigeront représenté respectivement 19,44% et 33,33% des espèces fongiques isolées en culture. Conclusion. L'otomycose est une infection fréquente en Orl à Niamey. Le nettoyage de l'oreille au coton tige ou à la plume de volaille venait au premier rang des facteurs favorisant l'otite fongique. L'Aspergilus nigeret le Candida albicansétaient les germes les plus fréquemment isolés.

The validity of the self-directed learning readiness instrument with the academic achievement among the Saudi medical students

Background: Self-directed learning (SDL) is very crucial for continuous professional development for all health-care professionals. Objectives: This study aims to measure the SDL readiness of the first clinical year medical students and its relation with their academic achievement. In addition, to examine the reliability and validity of SDL rating scales. Materials and Methods: This is a quantitative cross-sectional study involving the 3rd year medical students at a Saudi medical school. The level of SDL readiness was measured by a validated Fisher’s scale which includes three subscales: Self-control (SC), self-management (SM), and desire for learning (DL), and student’s academic achievement by their grade point average (GPA). The data were collected through a self-administered questionnaire and were analyzed using the Statistical Package for the Social Sciences. Results: High SDL scores were found to have a positive relationship with students’ academic achievement. The overall mean score of Fisher’s scale was 156.73 ± 15.85. The mean score of SC was 61.2 ± 6.4 followed by the DL 48.4 ± 5.6 and SM skills 47.1 ± 6.6. Factor analysis revealed that all three-factor models and individual subscales have an average to poor fit. Conclusion: The study results showed that the high SDL readiness score is directly proportional to students’ academic achievement. The students were highly self-directed learners, but the study could not confirm that the current SDL model had a strong level of reliability and validity when tested with undergraduate level medical student’s achievement by the GPA. Although the study confirmed that our students are self-directed learners, their time management skills need to be improved.

Dyslipidemia in young adults aged [20-40] years attending Baghdad teaching hospital and Al-Mansour primary health care center in Baghdad city

Hyperlipidemia is a condition characterized by high quantities of lipids in the blood stream. Lipids include cholesterol, triglycerides and phospholipids. Fats and cholesterol are generally processed in the liver and carried on proteins in the blood, forming lipoproteins. Its complications include pancreatitis, atherosclerosis, coronary artery diseases and stroke. Is to estimate the prevalence of hyperlipidemia in the Iraqi young adults aged 20-40 years and to shed a light on any significant association between some socio-demographic and life style variables with hyperlipidemia. A cross-sectional study was conducted from the first of December 2011 to the first of June 2012. The prevalence rate of any lipid abnormality [at least one abnormality] was 75%.The prevalence rates of elevated serum total cholesterol, low serum high density lipoprotein cholesterol, elevated serum triglyceride and elevated serum low density lipoprotein cholesterol were [32.5%, 38.5%, 29.5%, 30%], respectively. The prevalence rate of elevated TC/HDL ratio was 61%. There is a high prevalence of lipid abnormality in young adults of whom males had higher prevalence than females but the difference was not significant. The significant associated risk factors with hyperlipidemia were age, BMI, newly diagnosed or uncontrolled diabetic and hypertensive patients, cholesterol/fat rich diet and type of cooking oil

Sphincter saving surgeries for locally advanced low rectal cancer after neoadjuvant chemoradiation

Rectal cancer accounts for the largest distribution within one anatomical region of the large bowel, with approximately one third of all CRC located within the rectum. The Golden standard treatment of primary rectal cancer is curative surgical resection; however, affine balance remains between disease cure and restoration of gastrointestinal continuity. Combined modality has proven efficacy in many malignant tumors with advantage of organ preservation. Forty nine [49] patients with low rectal carcinoma were included in a prospective study, between Jan 2007 and Jan 2012. Preoperative chemoradiation was administrated to all patients and subjected to different techniques of sphincter saving surgery. Stage I and Stage IV disease at diagnosis were excluded from the study. Forty nine patients were included in the study. 27 [55%] patients were male and 22 [45%] were female the age range from 23 years to 70 years with the median age 46 years. The main presenting symptoms were bleeding per rectum and tenesmus. Stage II 18 patients [36.7%,], stage III 31 patients [63.3%. Complete clinical and pathological response in 3 patients [6%], and complete clinical response with only microscopically residual carcinoma in 20 patients [41%], partial response in 18 patients [36.7%], and no significant response in 8 patients [16%] 7 from 8 were mucoid carcinoma. Low anterior resection [LAR] in 22 patients [44.9%], Hartman 's procedure in 4 patients [8.1%], Coloanal pull-through [COP] was done in 19 patients [38.9%] and perineal colostomy in 4 patients [8.1%]. For patients with cola-anal pull-through technique complete dehiscent and retraction observed in 2 cases, Major leakage in one case, stenosis in 4 cases. There is tendency of colorectal cancers to affect younger groups. Most patients presented in advanced stage. Neadjuvant chemo radiation is an excellent tool in sphincter saving surgery. Coloanal pull- through technique is not wide spread technique for low rectal cancer with good oncological safety and acceptable functional outcome

effect of an organophosphorous insecticide on the hepatic, renal and pulmonary tissues of mice fetuses

The present study deals with the effects of the organophosphorous insecticide curacron on the hepatic, renal and pulmonary tissues of mice fetuses. Fourty pregnant females were divided into four groups, 10 individuals each. The 1st group served as control group. The individuals of the 2nd and 3rd groups were given oral dose of curacron 14.4 and 28.8 mg/kg, respectively on day 7 of gestation. The 4th group was given 14.4 mg/kg for 8 successive days from day 7 to day 14 of gestation. At the 18th day of pregnancy, females from both the control and treated groups were sacrificed and samples of hepatic, renal and pulmonary tissues were taken from the fetuses. The liver of the 18-days-old fetuses of all experimental groups showed different phases of venous congestion, disorganization of the hepatic lobules, fatty degeneration and necrosis. The kidney of curacron-treated groups showed venous congestion, cloudy swelling, and necrosis of the epithelial cells lining the kidney tubules. Moreover, the lungs of the different treated groups showed dilation and congestion of the blood vessels, thickening of the interalveolar septa and scattered cell debris in the lumens of the alveoli. Consequently, cumulative doses of curacon obviously affect hepatic, renal and pulmonary fetal tissues and extreme caution should be considered by pregnant women to avoid its hazardous effects on their fetuses