ABSTRACT
Tenascin is suggested the one of cause of vitiligo by interfering melanocyte adhesion and migration. The distribution and expression levels of tenascin were examined by semiquantitative immunohistochemistry on skin biopsies from vitiligo patients with varying area of photodamage. The level of tenascin on adult skin is severely restricted but we had observed the increase on vitiligo skin lesions. Although it is uncertain that the increased tenascin expression is the cause or the result of disease, vitiligo and increased tenascin expression is thought to be related each other. This study has shown that tenascin increased on photodamaged vitiligo skin lesions. So we shoud consider choosing phototherapy to vitiligo.
Subject(s)
Adult , Humans , Biopsy , Immunohistochemistry , Melanocytes , Phototherapy , Skin , Tenascin , VitiligoABSTRACT
Porokeratotic eccrine ostial and dermal duct nevus is considered to be a rare congenital hamartoma of eccrine duct origin. Histopathologic studies showed multiple cornoid lamella-like parakeratotic columns, which were associated with hyperplastic eccrine ostia and distal sweat ducts. I describe the case of a 20-year-old man with childhood-onset porokeratotic eccrine ostial and dermal duct nevus. He had a 10-year history of multiple keratotic papules and comedo-like pits on the thenar area and thumb of the right hand. But in my case, immunohistochemical studies for carcinoembryonic antigen showed no association with eccrine ostium and adjacent cornoid lamella-like structures.
Subject(s)
Humans , Young Adult , Carcinoembryonic Antigen , Hamartoma , Hand , Nevus , Sweat , ThumbABSTRACT
Prostatic carcinoma is one of the most common malignancies in males of western countries but it is a rare malignancy in Korea. If it occurs, it commonly metastasizes to other organs. In-spite of its high incidence of distant metastasis, the cutaneous metastasis of the prostatic carcinoma is very rare and seems to have a poor prognosis. A 67-year-old man presented multiple nontender nodules on the skin of the right thigh and had episodes of multiple metastases from prostatic carcinoma to the lungs and bones. Histopathologic findings of the skin showed atypical cells infiltration at the dermis. Immunohistochemical staining for prostate specific antigen showed a positive reaction, therefore, we reached the diagnosis of cutaneous metastasis from prostatic carcinoma.
Subject(s)
Aged , Humans , Male , Dermis , Diagnosis , Incidence , Korea , Lung , Neoplasm Metastasis , Prognosis , Prostate-Specific Antigen , Skin , ThighABSTRACT
The Carneys complex is a multisystemic tumorous disorder that features myxoma(heart, skin, and breast), spotty skin pigmentation(cutaneous and mucocutaneous), endocrine tumors(adrenal, testicular, and pituitary) and peripheral nerve tumors. The criteria far diagnosis of the complex is the presence of two or more of the above conditions. The condition is transmitted as an autosomal dominant trait. The most serious components of the syndrome are cardiac myxoma and psammomatous melanotic schwannoma. The cutaneous manifestations are a major clue in the disorder. We report a case of Carneys complex in a 19-year-old woman. She had spotty pigmentation on her face, left atrial myxoma, and myxoma on her right nipple. Her mother and sister had left atrial myxoma.
Subject(s)
Female , Humans , Young Adult , Diagnosis , Mothers , Myxoma , Neurilemmoma , Nipples , Peripheral Nervous System Neoplasms , Pigmentation , Siblings , SkinABSTRACT
BACKGROUND: Radiation has been used in t,he medical field of dragnosis and treatment. There is widely used ionizing radiat:ion such as naturally occuring r-rays or machine-made X-ray. This radiation is able to induce the structural and functional alterations of the mammalian cells. But we have few detailed reports on the effects of radiation on epidermal cells and their immune functions. OBJECTIVE: This study was performed to investigate the effect of radiation on cultured human keratinocytes and melanocytes. MATERIALS AND METHODS: Cultured human keratinocytes and melanocytes were irradiated with 2,6, l0Gy from a Co saurce and stimulated by 100 U/ml of ekratinocyte immediately after irradiation. We investigated cell numbers and morphological changes, DNA synthesis and HLA-DR antigen expression. RESULTS: After exposure to r-ray, the proliferation of keratinocytes and melanocytes decreased in a time and dose dependent fashion to each control group. Tliey showed decreased density, a larger size and a round appearance after radiation exposure and an especially shortened and decreased number of dendrites in the melanocytes. In DNA synthesis counted using [H]-thymidine incorporation, the keratinocvtes decreased values in a dose depen(lent manner at 24 and 72 hours after irradiation but no differense was observed at 168 hours. In melanocytes, there was a greater decrease than that of keratinocytes. The melanin content/cell in all radiation exposed groups increased in a time and dose dependent fashion t,o each contr ol group. HLA-DR antigen expression on keratinocytes after radiat,ion exposure decreased to the control group, but there were no significant differences acccirding to the dose of radiation, And there were no significant diifferences of HLA-DR antigen expression on the melanocytes betweer. controls and the radiation exposed groups. CONCLUSION: Antiproliferative activity was dependent on the exposure time and dose of r-ray exposure. According to the time after radiation exposure, melanogenic activity was stimulated. The expression of HLA-DR, antigen decreased in keratinocyte after radiation exposure but there was no decrease in melanocytes.
Subject(s)
Humans , Cell Count , Dendrites , DNA , HLA-DR Antigens , Keratinocytes , Melanins , MelanocytesABSTRACT
BACKGROUND: Chrome has been known to be a toxic and poten sensitizer and is contained in materials with which we are in frequent contact. But we have few reports detailing chrome contact dermatitis by clothes or the chrome content of materials in out use. OBJECTIVES: Our purpose was to evaluate the chrome content in rassieres. METHODS: We analyzed chrome in brassieres by using atomied absorption spectrophotometer, quantitatively and diphenylcarbazide method, qualitatively. RESULTS: The content of chrome in a 24 hour saline extracted g roup, a 48 hour saline extracted group and a pure nitric acid extracted group in the black was more abundant than in the reds or in the whites. But there was no aignificant difference in the different fabrics of the blacks. CONCLUSION: Although, in reality, it is hard to change chrome to an other substrate as the mordant of dyes, a limitation needed to prevent diseases induced by chrome during the process of dyeing. If someone has a sensitivity to chrome, it is necessary to wash new clothes before waring and to avoid black colored underwear.
Subject(s)
Humans , Absorption , Black People , Coloring Agents , Dermatitis, Contact , Diphenylcarbazide , Keratinocytes , Melanocytes , Nitric AcidABSTRACT
BACKGROUND: Radiation has been used in t,he medical field of dragnosis and treatment. There is widely used ionizing radiat:ion such as naturally occuring r-rays or machine-made X-ray. This radiation is able to induce the structural and functional alterations of the mammalian cells. But we have few detailed reports on the effects of radiation on epidermal cells and their immune functions. OBJECTIVE: This study was performed to investigate the effect of radiation on cultured human keratinocytes and melanocytes. MATERIALS AND METHODS: Cultured human keratinocytes and melanocytes were irradiated with 2,6, l0Gy from a Co saurce and stimulated by 100 U/ml of ekratinocyte immediately after irradiation. We investigated cell numbers and morphological changes, DNA synthesis and HLA-DR antigen expression. RESULTS: After exposure to r-ray, the proliferation of keratinocytes and melanocytes decreased in a time and dose dependent fashion to each control group. Tliey showed decreased density, a larger size and a round appearance after radiation exposure and an especially shortened and decreased number of dendrites in the melanocytes. In DNA synthesis counted using [H]-thymidine incorporation, the keratinocvtes decreased values in a dose depen(lent manner at 24 and 72 hours after irradiation but no differense was observed at 168 hours. In melanocytes, there was a greater decrease than that of keratinocytes. The melanin content/cell in all radiation exposed groups increased in a time and dose dependent fashion t,o each contr ol group. HLA-DR antigen expression on keratinocytes after radiat,ion exposure decreased to the control group, but there were no significant differences acccirding to the dose of radiation, And there were no significant diifferences of HLA-DR antigen expression on the melanocytes betweer. controls and the radiation exposed groups. CONCLUSION: Antiproliferative activity was dependent on the exposure time and dose of r-ray exposure. According to the time after radiation exposure, melanogenic activity was stimulated. The expression of HLA-DR, antigen decreased in keratinocyte after radiation exposure but there was no decrease in melanocytes.
Subject(s)
Humans , Cell Count , Dendrites , DNA , HLA-DR Antigens , Keratinocytes , Melanins , MelanocytesABSTRACT
BACKGROUND: Chrome has been known to be a toxic and poten sensitizer and is contained in materials with which we are in frequent contact. But we have few reports detailing chrome contact dermatitis by clothes or the chrome content of materials in out use. OBJECTIVES: Our purpose was to evaluate the chrome content in rassieres. METHODS: We analyzed chrome in brassieres by using atomied absorption spectrophotometer, quantitatively and diphenylcarbazide method, qualitatively. RESULTS: The content of chrome in a 24 hour saline extracted g roup, a 48 hour saline extracted group and a pure nitric acid extracted group in the black was more abundant than in the reds or in the whites. But there was no aignificant difference in the different fabrics of the blacks. CONCLUSION: Although, in reality, it is hard to change chrome to an other substrate as the mordant of dyes, a limitation needed to prevent diseases induced by chrome during the process of dyeing. If someone has a sensitivity to chrome, it is necessary to wash new clothes before waring and to avoid black colored underwear.
Subject(s)
Humans , Absorption , Black People , Coloring Agents , Dermatitis, Contact , Diphenylcarbazide , Keratinocytes , Melanocytes , Nitric AcidABSTRACT
BACKGROUND: Psoralen has been used in the treatment of certain hypojigmentary disorders with UVA or solar irradiation. However trecent report proposed the actions of psiralens are direct and do not require the presence of ultraviolet light. The report also suggested that tze specific receptors other than DNA would be present. OBJECTIVE: This study was done ta identify the effects of 8-methoryporalen(8-MOP) on the proliferation and melanization of cultured normal human melanocytes without UVA. METHODS: Melanocytes were cultured in melanocyte culture medium neluding 16% or 5% FBS. We added 8-MOP by their concentrations from 10 M to 10 M. After 8 hours treatment, we investigated the melanocytes proliferation and Lhe melanin contents. RESULTS: We could not detecet any significant differences of melanoytes proliferation and melanin contents between the control end experimental groups. CONCLUSION: There were no effect on the proliferation and the milanization of cultured normal human melanocytes with 8-MOP only.