ABSTRACT
Background: Diabetes mellitus is a major endocrine disorder and growing health problem in most countries. Diabetes manifested by experimental animal models exhibits high oxidative stress due to persistent and chronic hyperglycemia which increases the generation of free radicals, streptozotocin [STZ] provides an animal model of type 1 diabetes. Thereby depleting the activities of antioxidative defense systems with alteration of antioxidant activities of enzymes such as green tea and curcumin
Aim : Biochemical histological and histochemical investigations were carried on to revel the effect of STZ on the liver and pancreas cells. Natural antioxidants were used as a new way for ameliorating diabetic effect on the cells
Material and methods: Diabetes was induced by a single intraperitoneal injection of freshly prepared STZ dissolved in 0.05M of sodium citrate buffer, pH = 4.6, [STZ; 45 mg/kg B.wt.].Three days after degeneration of beta cells, diabetes was induced in all animals. After induction of diabetes, diabetic and normal animals were kept in metabolic cages separately. Green tea [EGCG] and curcumin are used as natural antioxidants to improve the disorders and structural changes induced by STZ. Cellular and histochemical investigations were carried on the changes induced in the pancreatic and hepatic tissues. Body weight, level of serum glucose and insulin were calculated in the control and treated groups. For detecting the degeneration of both hepatocytes and pancreatic cells of diabetic rats, tissue samples from diabetic and treated rats were collected and pathologically examined
Results: The present investigations reveled that there was a detectable amelioration on the injures induced by STZ on both hepatocytes and pancreatic cells using green tea or curcumin with a detectable dose level. Also it can be observed that the ameliorated effect induced was a time dependant. Conformation of these results from histochemical detection of polysaccharides and DNA contents were detected by PAS and Feulgen reactions
Conclusion: Curcumin and green tea look to have a powerful effect against diabetic cell injury induced in both rat liver and pancreas. The ameliorating effect seem to be time dependant
ABSTRACT
Cancer has become an important topic in medicine since it is a major cause of death in the developing countries and it is now only secondary to that of myocardial infraction. Nitrosamines precursors are known to be carcinogenic to humans, in various organs at relatively low levels of exposure. It induces benign and malignant tumors especially liver tumors following its administration by various routes, including ingestion and inhalation. Humans may be exposed to Nitrosamines through the ingestion of food that contains it, such as cured meat products, and smoked fish. Other exposures to Nitrosamines may be from drinking contaminated water and from breathing cigarette smoke and contaminated ambient air. Individual are most likely to be exposed to Nitrosamines in occupational settings such as in the rubber, tannery, fish processing, dyes, and surfactant industries. The prevalence of liver tumors throughout the world makes in imperative to seek chemo preventive agents. Vegetables, natural products of plant origin and numerous non-nutritive dietary constituents have been shown to play a salutary role in cancer chemoprevention. The present study aims to evaluate the chemo preventive efficacy of soy bean and Nigella sativa on hepato-carcinogenesis induced by dibuty1 nitrosamine [DEN]. It could be observed that both soy bean and Nigella sativa have a good effect of amelioration against liver hepatoma induced by nitrosamine. Soy bean more or less showed more prerogative effect than Nigella 9 and 12 months after administration