Protective effect of rosemary on acrylamide motor neurotoxicity in spinal cord of rat offspring: postnatal follow-up study / 대한해부학회지

The direct interactive effects of rosemary and acrylamide on the development of motor neurons in the spinal cord remains unknown. Our goal is to confirm the protective effects of rosemary against motor neuronal degeneration induced by acrylamide in the developing postnatal rat spinal cord using a postnatal rat model. We assigned the offspring of treated female rats into control, rosemary; acrylamide group; and recovery groups. This work depended on clinical, histopathological, morphometrically, immunohistochemical and genetic methods. In the acrylamide group, we observed oxidation, motor neuron degeneration, apoptosis, myelin degeneration, neurofilament reduction, reactive gliosis. Whoever, concomitant rosemary intake and withdrawal of acrylamide modulate these effects. These findings proof that dietary rosemary can directly protect motor neuron against acrylamide toxicity in the mammalian developing spinal cord.

Protective effect of vitamin E [c-tocopherol] on nicotine toxicity of the rat testis: histological, molecular and cytogenetic study

The harmful effects of nicotine on male fertility have been reported in experimental and clinical studies. However, the protective effect of vitamin [vit.] E against this toxicity is still a matter of controversy. To investigate a short time toxic effect of nicotine on the rat testis and to determine the possible protective role of vii. E [alpha-tocopherol] against this toxicity. Fifty four male albino rats were maintained for 15 days as follows: Group I, control group that divided into negative and positive control; group II, nicotine group [1mg/kg/day, intrapertonial [ip]] and group III, nicotine + vit. E [75mg/kg/day, intragastric [ig]. At the end of experimental study, testicular tissues and bone marrows were taken for histological, molecular and cytogenetic studies. Comparing with the control it was found that, simultaneous administration of vit. E and nicotine showed protective effect of vit. E against: Flistotoxicity, genotoxicity and mutagenicity of the nicotine, through increase in the thickness of germinal epithelium with significant [P<0.05] increase in the diameter of the seminilerous tubules, decrease in the nucleic acid destruction and significant decrease [P<0.05] in chromosomal aberrations, respectively. The present results seem to be rather surprising in view of the fact that nicotine could destroy testicular tissues in a short time duration and vit. E could produce partial protection through its antioxidant mechanism. Further studies are necessary to illuminate the other dark sides of nicotinic infertility in human spermatozoa and to discover others protective agents against its toxicity