ABSTRACT
Solute Carriers (SLC) superfamily is one of the most important membrane transporter families in the cell membrane (including intracellular membrane) of human,it is involved in some essential physiological functions such as intercellular substance transporting,energy transfer,nutrition and metabolism,signal transduction and so on.Solute Carriers (SLC) superfamily contains 52 subfamilies,with more than 400 members.Studies have shown that abnormal protein expression or functional defects of SLC caused by human genetic mutations are closely related with a variety of serious diseases such as diabetes,hypertension and depression,so the function research of SLC had attracted much attention in recent years.The known three-dimensional structures of SLC transporters help to explain the precise molecular mechanism of substrate binding and transporting,it provides a fine structure basis to study the molecular mechanism of related diseases and structure-based drug discovery.In this review,we summarized the progress on the structure and function of SLC superfamily transporters of late years,try to provide some common rules of SLC superfamily transporters.
ABSTRACT
Aim To study the anti-itching and anti-hypersensitivity effects of Doxepin hydrochloride cream. Methods The effects of Doxepin hydrochloride cream on the pruritus of skin in mice due to 4-AP and guineapigs due to histamine phosphate were observed. The inhibitory actions of it on the increase of abdominal cavity capillary permeability in mice due to acetic acid and the angiectasis of skin in rats due to histamine were also observed. Its effects on the delayed-type hypersensitivity (DTH) of mice due to 2, 4-dinitrochlorobenzol (DNCB) and the homologous passive anaphylaxis of skin in rats were used to investigate its anti-hypersensitivity actions. Results The application of Doxepin hydrochloride cream (50, 125, 250 mg?kg -1) on the skin of mice had significant inhibitory actions on the pruritus of skin caused by 4-AP, and the application of Doxepin hydrochloride cream (10, 25, 50 mg?kg -1) on the skin of guinea pigs also had significant inhibitory actions on the pruritus of skin caused by histamine phosphate. The application of Doxepin hydrochloride cream (10,25,50 mg?kg -1 and 50,125,250 mg?kg -1) on skin inhibited the increase of skin capillary permeability in rats caused by histamine phosphate and the increase of abdominal cavity capillary permeability in mice caused by acetic acid. The application of Doxepin hydrochloride cream (50, 125, 250 mg?kg -1 and 10, 25, 50 mg?kg -1) on skin also significantly inhibited the delayed-type hypersensitivity in mice due to 2, 4-dinitrochlorobenzol and the homogeneous cutaneous anaphylaxis in rats. Conclusions Doxepin hydrochloride cream can relieve itching,inhibit the increase of capillary permeability caused by inflammatory substances. It also has significant inhibitory actions on TypeⅠ and Type Ⅳ allergic reactions.