ABSTRACT
Objective:To explore the clinical charecteristics, imaging features, therapy and prognosis of stroke in children, and provide help for clinical treatment.Methods:The clinical data of 49 children with stroke were collectedand retrospectively analyzed in the Children′s Hospital of Soochow University from January 1, 2019 to December 31, 2019.Results:A mong the 49 children with stroke, 35 were male and 14 were female, aged 1-178 (65.69 ± 55.22) months; the specific etiologies were cerebrovascular malformation, craniocerebral trauma, tumor, vitamin K deficiencies, infectious diseases, rheumatic immune diseases, hemophilia and congenital heart disease. The first symptoms of stroke were disturbance of consciousness, hemiplegia, convulsions, vomiting and headache. The arterial ischemic stroke (18 cases) were mainly caused by craniocerebral trauma and cerebrovascular malformation. The hemorrhagic stroke (31 cases) were mainly caused by arteriovenous malformation, vitamin K deficiency and tumor. The surgical rate in the arterial stroke group was significantly lower than that in the hemorrhagic stroke group.Conclusions:Traumatic cerebral infarction and intracranial arteriovenous malformation are the main causes of arterial ischemic stroke and hemorrhagic stroke in children. Early diagnosis and treatment can significantly improve prognosis.
ABSTRACT
Objective To analyze the clinical characteristics of children with low grade glioma (LGG) and evaluate their survival status and quality of life in LGG pediatric patients after postoperative radiotherapy.Methods Clinical data of 27 LGG children aged ≤ 14 years admitted to Department of Radiation Oncology of our hospital from January 2011 to December 2017 were retrospectively analyzed.The 5-year overall survival (OS) and progression-free survival (PFS) were estimated with the Kaplan-Meier method.The quality of life was evaluated by body mass index (BMI),WeeFIM and enrollment situation.Results The 5-year OS and PFS of 27 pediatric patients were 92％ and 87％.BMI was measured in 25 children who were still alive and the results showed that 1 child was slightly emaciated,3 were overweight and 21 were normal.The total score of WeeFIM was＞ 108 in 21 cases and＜ 90 in 4 cases.In terms of social role-playing assessment,5 cases delayed enrollment for≥ 2 years,2 cases had good adaptation except the difficulties due to lameness,7 of them had poor study performance,but had no communication barrier.Conclusions Children with LGG can obtain relatively excellent OS and PFS after postoperative radiotherapy.Part of the children with long-term survival may experience a declined quality of life,which is mainly manifested with a decline in motor and cognitive functions.
ABSTRACT
Objective@#To analyze the clinical characteristics of children with low grade glioma (LGG) and evaluate their survival status and quality of life in LGG pediatric patients after postoperative radiotherapy.@*Methods@#Clinical data of 27 LGG children aged≤14 years admitted to Department of Radiation Oncology of our hospital from January 2011 to December 2017 were retrospectively analyzed. The 5-year overall survival (OS) and progression-free survival (PFS) were estimated with the Kaplan-Meier method.The quality of life was evaluated by body mass index (BMI), WeeFIM and enrollment situation.@*Results@#The 5-year OS and PFS of 27 pediatric patients were 92% and 87%. BMI was measured in 25 children who were still alive and the results showed that 1 child was slightly emaciated, 3 were overweight and 21 were normal. The total score of WeeFIM was> 108 in 21 cases and< 90 in 4 cases. In terms of social role-playing assessment, 5 cases delayed enrollment for≥ 2 years, 2 cases had good adaptation except the difficulties due to lameness, 7 of them had poor study performance, but had no communication barrier.@*Conclusions@#Children with LGG can obtain relatively excellent OS and PFS after postoperative radiotherapy. Part of the children with long-term survival may experience a declined quality of life, which is mainly manifested with a decline in motor and cognitive functions.
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new glioma cell line and analyze its biological characteristics, and to provide a useful cellular tool with new features for cancer research.</p><p><b>METHODS</b>Glioma tissue was taken from surgical specimen clinical of a clinical patient. Primary culture was carried out, and a cell line (SHG139) was established after 10 passages. Immunofluorescence staining was performed to detect the expression of proteins, and cell proliferation and cycle were detected by flow cytometry method (FCM). The biological characteristics of SHG139 cells were detected by chromosome karyotype analysis. SHG139s glioma cells derived from SHG139 glioma cell line were cultured with neural stem cell medium. Then stem cell markers were determined. SHG139s cells were induced with serum-containing medium, and their expression of A2B5, GFAP, β-III tubulin, and GalC was detected. Intracranial xenograft tumor of both SHG139 glioma cells and SHG139s glioma stem cell spheres was generated in rats.</p><p><b>RESULTS</b>The expressions of A2B5, GalC, GFAP, S-100, and vimentin in the 20 and 60 passages of SHG139 cells were positive, consistent with the immunohistochemical results and pathological features. SHG139 cells proliferated significantly within 24 h after subculture, and their total number of chromosomes was 68 and mostly multiploid. They were positive for A2B5 (84.12±9.96)%, nestin (73.86±5.01)%, and NG2 (73.37±2.09)%. SHG139s cells were induced, and the ratio of positive cells of GFAP, β-III tubulin and GalC was (92.89±2.24)%, (64.85±4.09)% and (33.57±4.14)%, respectively.</p><p><b>CONCLUSIONS</b>SHG139 is an astroglioma cell line, from which SHG139s cells can be successfully obtained by culture with NSCM. SHG139s cells are of A2B5(+)/CD133(-) GSCs subgroup cells, with potentials of self-renewal and multi-directional differentiation. Compared with the intracranial SHG139 xenograft tumor, the intracranial SHG139s xenograft tumor is more malignant and aggressive.</p>