effect of stressful exercise and / or local implants of labeled Buffy coat cells on muscle regeneration

The process of muscle regeneration is an important field in scientific work Thus. An attempt has been carried out to find the link between the most important factors which may affect this process and these are exercise and local buffy coat implantation. 168 male albino rats were divided into 5 groups. All of these groups were exposed to unilateral calf muscle injury of their right legs. The 1[st] group was regarded as control, the 2[nd] was exposed to stressful exercise, the 3[rd] was exposed to local implantation of labelled autogenous buffy coat cells at the site of muscle injury, the 4[th] was exposed to both events while the 5[th] was exposed to local injection of the labelling material at the injury site. Follow up of the process of muscle regeneration had been carried out for 28 days. Results have shown that muscle regeneration was enhanced in the 2[nd] and 4[th] groups where stressful exercise had been performed, possibly by providing myoblast precursors from the circulation and / or increasing myoblast spillage into the area by multiple fiber disruptions . Buffy coat had also enhanced muscle regeneration by transformation of some of them into myoblasts as indicated by the presence of labelled cells in the regenerated muscle tissue in the 3[rd] and 4[th] groups. These findings had led to the conclusion that, muscle regeneration can be improved by using stressful muscle exercise together with local implantation of buffy coat cells at the site of muscle injury. An intimate relation had been predicted between buffy coat implantation, stressful exercise and muscle regeneration

demonstration of B-esterase in the dorsal gery horn of the rats spinal cord during pain and analgesia

The B-esterase technique has been utilized as a cheaper and less sophisticated technique than the immuno-histochemical method for the demonstration of substance P prevalence sites at the dorsal grey horn of the spinal cord of rats. B-esterase reaction product was abundant at laminae I and II of dorsal grey horn during the induction of pain while analgesia reduced the activity of this enzyme at the same sites

histochemical study X-irradiation of the rabbit lens and its treatment

Na-K-ATPase and carbonic anhydrase [CA] activities in rabbit lens were studied histochemically at various times after exposure to a single dose of 2000 rads of X-ray, and were compared with their activities in the contralateral control lens. In the X-irradiated lenses, an increase in the Na-K-ATPase and CA activities was observed at the third and fourth weeks after exopsure, respectively. This was followed by a reduction in Na-K ATPase activites at the fourth week, and CA at the Twelfth week [prior to development of opacity at the thirteenth week]. The effect of topical applications of two proposed substances, horseradish peroxidase [HRP] as an antioxidant and honey as an anecdotal drug were studied clinically and histochemically in the X-irradiated rabbit lenses. Both drugs, particularly HRP. were able to delay or prevent changes due to X-irradiation. The histochemical verdict and the possible role of these substances are discussed in term combating the free radicals formed

Treatment of advanced chorionic carcinoma with indomethacin and steroids: case report

A patient with advanced chorionic carcinoma was treated with cytotoxic drugs, methotrexate and 6-mercaptopurine for a period of three months. Her general condition, including physical, laboratory and radiological findings, deteriorated during and after treatment. She was then given 75 mg of indomethacin and 15 mg of prednisolone daily; signs of improvement, both clinical and radiological, were noted after four weeks of this treatment. It might be concluded that this therapy contributed successfully to the course of her disease and her well-being

applicability of NSE staining in hematological smears

Monocytes identification and count in haemotological smears were compared by using two stains: the first, was the Romanowsky stain used in routine work; the second, was an enzymatic one the nonspecific esterase [NSE] activity using alpha naphthyl acetate as a substrate. As the T-lymphocytes also show NSE activity with this substrate they were identified and counted as well. The NSE staining technique was found to be more reliable, Its applicability is discussed in this paper

effect of antigens and prostaglandin A1 on serum protein components during primary and secondary immune response

Rabbits immunized with a thymus dependent antigen [Ag] [sheep RBCs] and with a thymus independent Ag [E. coli O Ag], were treated with prostaglandin Al [PGA1] daily by subcutaneous [S.C] injection of 25 mg/kg body weight. The results showed that both Ags cause a number of changes on serum protein components [S.P.C.] 7, 14, and 21 days after primary immunization [P.i]. Most of these changes were statistically significant [S.S]. While PGAI, on the other hand, causes augmentations or diminusions of these changes. The same results were obtained 10 and 18 days after secondary immunization [S.i] with both Ags. Therefore, it seems that the changes on SPCs accompanying various chronic pathological conditions could be ascribed to interaction between prolonged antigenic stimulation and PGs release