ABSTRACT
Objective To study the therapeutic effects of blood component transfusions on acute leukemia complicated with DIC.Methods The blood component transfusions were mainly used to treat 11 patients with acute aranulocytic leukemia and acute lymphatic leukemia complicated with DIC.Results Three cases were cured,one case were obviously effective,two were improved and five were ineffective,which the total effective rate was 54 5% .Conclusion Early diagnosis and blood component transfusions in timely are effective methods to treat the acute leukemia patients complicated with DIC
ABSTRACT
To investigate the effects of granulocyte macrophage colony stimulating factor (GM-CSF) which was secreted from the fresh leukemic cells of patients with acute myeloid leukemia (AML) and recombinant human GM-CSF(rhGM-CSF) on the apoptosis of the primary incubative AML cells,the level of GM-CSF was measured by means of radioimmunoassay,the DNA ladder was detected by using DNA electrophoresis,and the percentage of apoptotic cells,percentage of expression and the mean fluorescence intensity(MFI) of bcl-2 were respectively assayed by means of flow cytomtric analysis.After the fresh leukemic cells from 5 cases were incubated for 24 hours,GM-CSF in 3 of 5 cases were seen,their percentage of apoptotic cells was less than the other 2 cases,and the MFI of bcl-2 in the 3 of 5 cases was obviously enhanced.After the fresh leukemic cells were treated with cytarabine (Ara-C) 5?g/ml in combination with rhGM-CSF 5?g/ml for 24 hours,the percentage of apoptotic cells was more than the cells treated with Ara-C alone and it upward to (56 4?7 4)%(p