ABSTRACT
The aim of the current study is to evaluate the effect of ethanolic seed extract of Caesalpinia bonduella (ESECB)on altered hormonal levels in mifebristone-induced polycystic ovarian syndrome (PCOS) female rats. PCOS wasinduced in female rats by a daily administration of mifepristone (4 mg/kg b.w.) for 8 days which was confirmed bythe persistent estrous cycle. Once PCOS was induced, the rats were treated with ESECB 200 and 400 mg/kg b.w.p.o. for 28 days, and the hormonal parameters were analyzed. Metformin was used as a standard drug. A significantincrease (p < 0.01) in the luteinizing hormone, Prolactin, insulin, testosterone, estrone, and estradiol levels anddecrease in follicle stimulating hormone and progesterone hormone levels in the PCOS rats were observed, whichwere normalized in the ESECB drug-treated animals. Among the two concentrations, 400 mg/kg b.w of ESECB drugtreatment showed significant effect in correcting this hormonal imbalance. Its effect was comparable to the positivecontrol used in the present study. It can be concluded that the ESECB can be used as a potential ovulation inducerin the treatment of PCOS
ABSTRACT
The combined Bi-herbal ethanolic extract (BHE) made up of equal quantities of leaves of Pisonia grandis and Cardiospermum halicacabum was evaluated for its synergistic in vitro and in vivo Anti-arthritic effects and was compared with its individual ethanolic extracts of Pisonia grandis (EPG) and Cardiospermum halicacabum (ECH). The inhibition of protein denaturation, membrane stabilization and proteinase enzyme inhibition were studied as in vitro and Freund’s adjuvant arthritis model in Wistar rats was used to study the in vivo Anti-arthritic activity. The three extracts (BHE, EPG, and ECH) at the dose level of 300mg/kg b.w.in 2%CMC were ingested orally to the CFA treated rats once daily for 40 days and the percentage inhibition of the paw volume increase due to the drug treatment was calculated. All the three plant extracts exhibited dose dependent stabilization of the RBC membranes, inhibition of protein denaturation and protease inhibition. The BHE has got a significant reduction in the paw volume and oedema when comparable to EPG and ECH in these intoxicant treated animals. These observed effects may be due to its inhibition of free radical production due to the immunological and inflammatory reactions or minimizing protein denaturation or proteinase enzyme or stabilizing the membranes.