Histopathological features and immunoflorescence patterns in skin lesions

The diagnosis of vesiculo-bullous lesions and many other diseases is fundamentally based on the microscopic study of cells and tissues. This diagnostic method remains the standard, by which all other diagnostic tests are measured. Nevertheless, the era of the pathologist relying entirely on the examination of tissue sections stained by histochemical methods is gradually being replaced by a time Sridhar Reddy Erugula, P. Sujatha, Jesudass Govada, B. Suresh Reddy, B. Kavitha Reddy, Oruganti Venkata Ramanand, Pothuraju Haritha, Kandukuri Mahesh Kumar. Histopathological features and immunoflorescence patterns in skin lesions. IAIM, 2016; 3(11): 171-183. Page 172 when advanced immunologic and molecular techniques (i.e. analysis of DNA, RNA or protein structure and function) augment the process by which complicated diseases are classified.

Method Development and Validation for Simultaneous Determination of Paracetamol and Ketoprofen by RP – HPLC Technique.

The present study describes a simple, accurate and precise RP-HPLC Technique for the simultaneous determination of Paracetamol and Ketoprofen in pharmaceutical dosage form. The method involves an isocratic elution of drug in a stationary phase of YMC, C4 (150 x 4.6 mm, 3 μm) column using a mobile phase composition of methanol and 0.1% (v/v) orthophosphoric acid in the composition ratio of 70:30 v/v with a flow rate of 0.9 mL/min at 254nm of detection. The injection volume is 10 μL. the method has been validated for specificity, linearity, range, precision, accuracy, limit of detection, limit of quantification, ruggedness and robustness. The retention times for Paracetamol and Ketoprofen are about 1.50 and 6.27 minutes respectively. Quantitative linearity was observed over the concentration range of 50 to 500 μg/mL for Paracetamol and 20.05 to 200.54 μg/mL for Ketoprofen respectively. The regression equations of concentration of Paracetamol and Ketoprofen are found to be y = 1319x+8671, y = 5280x + 29575 respectively where y is the peak area and x is the concentration of drug (μg/mL). The % recovery of Paracetamol and Ketoprofen are found to be in the range of 97% to104 %. All the validation parameters are within the acceptance range.

A detailed approach on multiple myeloma and its treatment.

Multiple myeloma is a cancer formed by malignant plasma cells. Normal plasma cells are found in the bone marrow and are an important part of the immune system. The immune system is composed of several types of cells that work together to fight infections and other diseases. Lymphocytes are the main cell type of the immune system. There are 2 major types of lymphocytes: T cells and B cells. When B cells respond to an infection, they mature and change into plasma cells. Plasma cells make the antibodies that help the body attack and kill germs. Lymphocytes can be found in many areas of the body, such as lymph nodes, the bone marrow, the intestines, and the bloodstream. Plasma cells, however, are mainly found in the bone marrow. Bone marrow is the soft tissue inside some hollow bones. In addition to plasma cells, normal bone marrow has cells that make the different normal blood cells. When plasma cells become cancerous and grow out of control they can produce a tumor called a plasmacytoma. These tumors generally develop in a bone, but they are also rarely found in other tissues. If there is only a single plasma cell tumor, it is called an isolated plasmacytoma. When there is more than one plasma cell tumor, it is called ‘multiple myeloma’. There are lot of plasma cell abnormalities, but multiple myeloma causes severe bone pain, so here we covered detailed notes on the disease and its treatment strategies.

Equilibrium and kinetic studies of methylene blue and rhodamineb onto prepared activated kaza’s carbons.

Three Activated Kaza’s Carbons viz. NPTC, NLLC andNCC, are prepared from some Bio- Materials – Phaseolus trilobus, Leucena leucocephala and Casurina. These carbonsare used to adsorb dyes Methylene blue and Rhodamine B from aqueous solution. Adsorption equilibrium data and kinetic processes were studied. The Langmuir isotherm provides the best correlation for the experimental data, where as Temkin and Dubinin – Radushkevich (D-R) isotherms also fit to the experimental data. In the kinetic studies the best fit Model was assessed by the squared some of errors (SSE) values. Adsorption of dyes, Methylene Blue ( MB ) and Rhodamine B ( RB ) ,on to adsorbents could be described by pseudosecond order equation. The adsorption process has good co-ordinance with pore diffusion and Elovich equations.