ABSTRACT
A new, simple, precise, accurate and robust high‐performance thin‐layer chromatographic (HPTLC) method has been developed for simultaneous estimation of β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A in an Ayurvedic formulation, Ashvagandharishta. The plates were developed in mobile phase consisting of toulene: ethyl acetate: formic acid: methanol (6:3:0.1:0.6, v/v/v/v). The retention factors of β-sitosterol-Dglucoside, gallic acid, withaferin A and withanolide A were 0.18 ± 0.01, 0.26 ± 0.02, 0.37 ± 0.02 and 0.57 ± 0.02, respectively. Linearity was in the range of 50‐400 ng/band for β-sitosterol‐D‐glucoside, 150-500 ng/band for gallic acid, 50‐450 ng/band for withaferin A and 50-500 ng/band for withanolide A. Accuracy study showed recoveries of 99.04 - 102.67, 98.77 - 101.96, 99.64 - 102.67 and 100.07 - 101.96 % for β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A, respectively. The results obtained in validation assays indicated accuracy and reliability of HPTLC method for simultaneous quantification of β‐sitosterol‐D‐glucoside, gallic acid, withaferin A and withanolide A in Ashvagandharishta. The percent content of β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A in Ashvagandharishta were found to be 0.012, 0.064, 0.016 and 0.312 %, respectively.
ABSTRACT
Aims: Aim of this study was to evaluate the comparative efficacy of Tinospora cordifolia (Willd.) Miers ex Hook. F., Tinospora sinensis (Lour.) Merrill and T. cordifolia growing on Neem (Azadirachta indica A. Juss.) called Neem-guduchi. Study Design: Selected species have been widely used in the traditional medicine systems in various dosage forms to treat liver disorders. They are of common occurrence and are being used as substitutes to each other. There is no comparative hepatoprotection study yet published, therefore, present study has carried out. Place and Duration of Study: Interactive Research School for Health Affairs, Pune and Amrutvahini College of Pharmacy, Sangamner, between November 2011 and August 2012. Methodology: Guduchi-Satwa, a well-known dosage form was prepared according to the traditional procedure. Hepatoprotective potential was assessed using paracetamolinduced hepatotoxicity model in rats and evaluated by using biochemical parameters viz. alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin (BIL). Results: Both T. cordifolia and T. sinensis Satwa significantly reduced the paracetamol induced elevated levels of serum ALT, AST, ALP and BIL at dose of 200 mg/kg, i.p. as compared to Neem-guduchi. Conclusions: Satwa preparation form of T. sinensis offers exploitable level of hepatoprotection potential.
ABSTRACT
Myrrh (guggulu) oleoresin from the Commiphora mukul tree is an important component of antiarthritic drugs in Ayurvedic medicine. Clinical data suggest that elevated levels of hyaluronidase and collagenase type 2 enzymes contribute significantly to cartilage degradation. Triphala guggulu (TG) is a guggulu-based formulation used for the treatment of arthritis. We assessed the chondroprotective potential of TG by examining its effects on the activities of pure hyaluronidase and collagenase type 2 enzymes. Triphala shodith guggulu (TSG), an intermediate in the production of TG, was also examined. A spectrophotometric method was used to assay Hyaluronidase activity, and to detect potential Hyaluronidase inhibitors. Aqueous and hydro-alcoholic extracts of TSG showed weak but dose-dependent inhibition of hyaluronidase activity. In contrast, the TG formulation was 50 times more potent than the TSG extract with respect to hyaluronidase inhibitory activity. A validated X-ray film-based assay was used to measure the gelatinase activity of pure collagenase type 2. Hydro-alcoholic extracts of the TG formulation were 4 times more potent than TSG with respect to collagenase inhibitory activity. Components of Triphala were also evaluated for their inhibitory activities on hyaluronidase and collagenase. This is the first report to show that the T2 component of Triphala (T.chebula) is a highly potent hyaluronidase and collagenase inhibitor. Thus, the TG formulation inhibits two major enzymes that can degrade cartilage matrix. Our study provides the first in vitro preclinical evidence of the chondroprotective properties of TG.
Subject(s)
Chromatography, Thin Layer , Collagenases/antagonists & inhibitors , Commiphora/chemistry , Enzyme Inhibitors/pharmacology , Herbal Medicine , Hyaluronoglucosaminidase/antagonists & inhibitorsABSTRACT
This is the first report describing two novel chondroprotective activities of aqueous extracts of Withania somnifera root powder.First,these extracts had a statistically significant,short-term chondroprotective effect on damaged human osteoarthritic cartilage matrix in 50% of the patients tested. Second,these extracts caused a significant and reproducible inhibition of the gelatinase activity of collagenase type 2 enzyme in vitro.