ABSTRACT
A simple, specific, accurate and isocratic reversed phase liquid chromatographic method was developed and subsequently validated for the determination of metformin hydrochloride, rosiglitazone and pioglitazone hydrochloride. Separation was achieved with a Zorbax C8 column of 150×4.6 mm i.d. with 5 μm particle size and ammonium dihydrogen phosphate buffer adjusted to pH 3.0 using diluted ortho phosphoric acid and acetonitrile (65:35 v/v) as eluent at a constant flow rate of 0.7 ml per min. UV detection was performed at 215 nm. The retention time of metformin hydrochloride, rosiglitazone and pioglitazone hydrochloride were about 1.9, 3.4 and 6.7 min, respectively. This method is simple, rapid and selective and can be used for routine analysis of antidiabetic drugs in pharmaceutical preparation. It is a convenient method for separation and simultaneous determination of metformin hydrochloride, rosiglitazone and pioglitazone hydrochloride in pharmaceutical formulations.