Improved Decipherment of the Protein Database of Human Proteins in the PDMD (Protein-Direct-Microsequencing-Deciphering) Method

Human proteins seem to be processed by Human serum biotinidase, and Human excreted proteins seem to be handled with by Human serum biotinidase and Human Chymotrypsin A. Therefore, we must improve PDMD method by using these new findings. Protein determination is performed by the highly sensitive HPLC-SEC-photometric method at UV 210 nm; i.e., c.a. 200-fold sensitive than Lowry抯 method. Human proteins are found to be not metabolized at membrane inserted portions. Membrane and Hydrophobic proteins of Humans are defined as the precipitable proteins at 100,000 x g for 90 min at 4 C, and have hydrophobicities larger than 0.515.

Weil's disease with elevated plasma D-dimer.

We report a case of Weil's disease manifesting elevation of plasma D-dimer in the acute phase. Later, in the convalescent phase after treatment, the plasma levels of D-dimer returned to normal. Plasma D-dimer can be used as a marker of fibrin formation and degradation. Based on our observations, we conjecture that fibrin formation and fibrinolysis may occur in the acute phase of Weil's disease.