Detection of AmpC and ESBLs beta -lactamase- among Klebsiella pneumoniae clinical isolates

This study was undertaken to determine the beta-lactamases which are cephalosporinases that confer resistance to a wide variety of beta - lactam drugs and that may thereby create serious therapeutic problems. Forty clinical isolates of Klebsiella pneumoniae recovered from different specimens collected from Kasr Al-Aini hospital, Cairo, Egypt were tested. Klebsiella pneumoniae showed 100% resistance against amoxicillin and penicillin, followed by ampicillin/sulbactam [75%], tobramycin [72.5%], cephradine and tetracycline [70%]. In contrast it showed 90% sensitivity and intermediate susceptibility against imipenem followed by azithromycin, which showed 67.5%, ofloxacin [55%] and levofloxacin [50%]. ESBL tests were performed using the recommended methods by Clinical and Laboratory Standards Institute [CLSI] as disc diffusion method and the phenotypic confirmatory test by combination disc method showing ESBL positive results in ration of 87.5% for the first test and 90% for the second confirmatory test. Sixty percent of isolates of Klebsiella penumoniae tested using boronic acids were of class C beta-lactamases producer. Detection of AmpC or ESBLs may aid in hospital infection control and the ability of the physician to prescribe the most appropriate antibiotic, thus decreasing the selective pressure, which generates antibiotic resistance

Resistance to imipenem in clinical isolates of kiebsiella pneumoniae and proteus mirabilis

Six Klebsiella pneumoniae and seven Proteus mirabilis clinical isolates were multiple drug resistant producing beta-lactamases. Out of the thirteen isolates, eight were resistant to third generation cephalosporin, cefotaxime and one was of intermediate resistance. Similarly, five isolates were resistant to fourth generation cephalosporin, cefepime with another five of intermediate resistance. However, isolates showed high alarming resistance pattern against imipenem and meropenem antibiotics. A representative imipenem resistant isolate from both strains were picked out for the detection of 40 kDa outer membrane protein and plasmid profile

Isolation and identification of urinary tract Infectious proteus mirabilis

Pr. mirabilis causes several infections in patients with 'complicated' urinary tract infections. In cases where stones are formed, the bacteria can become resistant to antibiotics. Pr. mirabilis can be diagnosed in the laboratory due to its characteristic swarming motility, and inability to metabolize lactose on a MacConkey agar plate. The identification and antibiotic sensitivity of hundred and two urine samples [38 males, 64 females] with urinary tract infection [UTIs] were investigated. Sixty samples, [59%] had associated urinary tract infection with Pr. mirabilis which has the most common causative microorganism. The bacterial isolates altered in their susceptibility to antimicrobial agents. Indole test, methyl red test, trytophan test, oxidase test, and casein test were appeared to be negative but the catalase test, phenylalanine deaminase test, urea test, hydrogen suflide test and citrate agar test were exerted a positive results