Effects of BCL6 B on proliferation and migration of human colorectal car-cinoma LoVo cells and its potential mechanism / 中国病理生理杂志

AIM:To detect the endogenous expression of B-cell leukemia/lymphoma 6 member B (BCL6B) in FHC and LoVo cells, and to investigate the effects of BCL6B on proliferation and migration of LoVo cells for further explo-ring the underlying mechanism .METHODS:The endogenous expression of BCL 6B in the FHC and LoVo cells was detec-ted by RT-PCR and Western blot .The methods of MTT assay , colony formation assay , wound healing assay and Transwell chamber experiment were employed to examine the biological functions of BCL 6B in the LoVo cells.The mRNA and protein levels of BCL6B, cyclin D1 and matrix metalloproteinase-9 ( MMP-9) were determined by RT-PCR and Western blot , re-spectively.The level of phosphorylated protein kinase B (p-AKT) was detected by Western blot.RESULTS:BCL6B ex-pression was notably repressed in the LoVo cells as compared with the FHC cells , which were significantly increased by transfection with pcDNA3.1-BCL6B.The abilities of proliferation and migration of the LoVo cells at 72 h were inhibited by 28.33%(P<0.01) and 36.11%(P<0.05) in BCL6B group.The mRNA levels of cyclin D1 and MMP-9 in the cells of BCL6B group were decreased by 39.90%(P<0.01) and 77.36% (P <0.05), and the protein levels of cyclin D1, MMP-9 and p-AKT were reduced by 44.00%(P<0.05), 47.06%(P<0.01) and 32.88% (P<0.05), respectively. CONCLUSION:BCL6B inhibits proliferation and migration of the LoVo cells , and the PI3K/AKT signaling pathway is in-volved in this process .

High-intensity focused ultrasound inhibits tumor metastasis in a melanoma-bearing mouse model / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of high-intensity focused ultrasound (HIFU) on tumor metastasis in mouse model bearing melanoma xenograft.</p><p><b>METHODS</b>Mice bearing murine melanoma B16-F10 cell xenograft were randomized for sham-HIFU or HIFU exposure when the tumors grew to a maximum diameter of 7-10 mm, and the tumor size was measured every 3 days. The cumulative survival rate of the mice and tumor metastasis rate were calculated, and the circulating melanoma cells were detected using qRT-PCR. At 14 days after HIFU treatment, B16-F10 cells were retransplanted via the tail vein and the pulmonary metastatic nodules were counted.</p><p><b>RESULTS</b>The median survival time of the mice was 19.00 days (95% CI 17.14-20.86 days) in the sham group and 26.00 days (95%CI 24.76-27.25 days) in HIFU group. The cumulative survival rate in the HIFU group was significantly higher than that in sham-HIFU group (P<0.01), and the tumor size was significantly smaller in HIFU group at 20, 23, and 26 days after HIFU treatment (P<0.05). Compared with the sham-HIFU group, HIFU group had significantly lower levels of MAGE-A3, MART1 and PAX3 at 7 days after HIFU (P<0.05) with still lower MAGE-A3 level at 14 days (P<0.05). HIFU group showed a significantly smaller number of pulmonary metastatic nodules following tumor cell retransplantation than in sham-HIFU group (P<0.01) with a metastasis inhibition rate of 42.4%.</p><p><b>CONCLUSION</b>HIFU treatment can inhibit tumor metastasis in melanoma-bearing mice possibly by reducing tumor cell detachment from the primary tumor site and suppressing colonization of the circulating melanoma cells.</p>

Time varied stress effects on the proliferation of myoblast in rats / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the effects of time varied stress on the proliferation of myoblast in rats and provide the basic experimental data for the remodeling of tissue in functional orthopaedics.</p><p><b>METHODS</b>Based on the pulsatile mechanical system founded, this study loaded different strain (2.5, 5.0, 10.0 kPa) to the myoblast of lateral pterygoid muscle. The proliferation of myoblast was detected by 3H-TDR.</p><p><b>RESULTS</b>After 6 hours under time varied strain, the significant proliferation of myoblast (P < 0.05) was observed, and the 5.0 kPa group expressed the best proliferation. After 12 hours under time varied strain, all groups expressed a better proliferation. Meanwhile, the lower frequency (0.40 Hz) had the bigger effect on the proliferation more than in the higher frequency (1.25 Hz) under the same time varied strain.</p><p><b>CONCLUSION</b>The frequency of time varied strain had also the important influence on the proliferation, the lower frequency (0.40 Hz) had the bigger effect on the proliferation more than in the higher frequency (1.25 Hz) under the same time varied strain. In the certain period of time and certain magnitude of time varied strain, the proliferation of myoblasts rised.</p>