ABSTRACT
<p><b>OBJECTIVE</b>To investigate fluorescence in situ hybridization labeled with quantum dots (QDs) for the detection of human papillomavirus 16/18 (HPV16/18) infection in cervical carcinoma patients.</p><p><b>METHODS</b>A total of 80 biopsy samples of squamous carcinoma of cervix were assayed for HPV 16/18 infection by using quantum dot labeled fluorescent in situ hybridization (QD-FISH) and chromogenic in situ hybridization (CISH) techniques, respectively. The results obtained by using two different methods were statistically analyzed.</p><p><b>RESULTS</b>The positive rate for HPV16/18 by QD-FISH was 88.8% (71/80), higher than that (80.0%) by CISH, however, the result was statistically not significant (P=0.127). The positive detection rates for HPV16/18 by using both methods increased coincidentally with raising of the tumor grading stage.</p><p><b>CONCLUSION</b>The sensitivity and specificity of HPV infection detectable by QD-FISH is higher than that by the CISH technique.</p>
Subject(s)
Adult , Female , Humans , Middle Aged , Carcinoma, Squamous Cell , Pathology , Virology , Uterine Cervical Dysplasia , Pathology , Virology , Chromogenic Compounds , Human papillomavirus 16 , Human papillomavirus 18 , In Situ Hybridization, Fluorescence , Methods , Papillomavirus Infections , Virology , Quantum Dots , Sensitivity and Specificity , Uterine Cervical Neoplasms , Pathology , VirologyABSTRACT
<p><b>OBJECTIVE</b>To analyze the relationship between DNA content and biological behavior and its prognostic significance in non-small cell lung cancer.</p><p><b>METHODS</b>Tumor DNA content was determined by flow cytometry in the specimens from 58 patients with resected non-small cell lung cancer. The DNA content of each cell subpopulation was expressed as the DNA index (DI), and an internal standard was provided by the normal pulmonary parenchymal cells in the same specimen. The prognostic value of DNA content in non-small cell lung cancer was assessed by Cox's model analysis.</p><p><b>RESULTS</b>In qualitative analysis, there was no relationship between DNA ploidy (diploidy or aneuploidy) and the following factors: tumor size, metastasis of lymph node, clinical stage, pathologic type, pathologic grade or survival. In quantitative analysis, high DNA index was observed in tumor size > 3 cm, metastasis of lymph node, stage III/IV, adenocarcinoma and shorter survival, which was statistically significant. Cox's model analysis showed that DNA index was a prognostic factor in non-small cell lung cancer and DNA index > 2.0 was an independent prognostic factor.</p><p><b>CONCLUSION</b>DNA index analysis is useful for the evaluation of the biological behavior and the prognosis of non-small cell lung cancer.</p>