ABSTRACT
Purpose: This study was undertaken to demonstrate histologically and histochemically the difference of bone healing using Equine Heterologous Bone versus Bovine Bone in induced bony cavities in the submental area of guinea pigs
Materials and Methods: Fifteen adult male guinea pigs with an average weight of 400-500 grams were used in this study. Each animal was subjected to implantation of the spongy granular form of Equine Heterologous Bone Tissue [Bio Gen] in an induced bony cavity through the right submental area of the mandible adjacent to the midline [study side]. A similar bony cavity was made on the left side [control side] adjacent to the midline in the same animals for implantation of Bovine Bone [Bio-Oss]. Animals were classified into 5 groups [3 animals each] according to the predetermined time of sacrifice; at 1 month [group I], 1.5 months [group II], 2 months [group III], 4 months [group IV] and 6 months [group V]. Bony specimens were decalcified in EDTA and processed for paraffin embedded sections [buccolingual sections of 4 micro thick] for histological and histochemical examination
Results: Both histological and histochemical results showed faster bone formation and maturity of study side osseous defects at all study periods than its counterparts of the control ones. In addition, the total proteins of the formed bone in the study side sections were less than the control side ones
Conclusion: The histological and histochemical results of this study revealed that Equine Bone Tissue was integrated and subsequently replaced by new bone formation with faster maturity than was the Bovine Bone