Identification of Lacrymaria velutina (Pers. Ex Fr.) Konrad & Maubl. from Micheon-myeon, Jinju-city, Korea

We identified Lacrymaria velutina of the Coprinaceae in Korea. The unusually large and sturdy fruiting body, fibrillose to fibrillose-scaly cap and stalk without a volva with an obscure superior hairy ring zone or hairy annulus, and blackish brown, warted spores distinguished this species from closely related Psathyrella species. An illustrated account of the microscopic traits is presented. Fruiting bodies with obtusely hemispherical caps, 2.5~6 cm, becoming convex with age; surface dry, densely fibrillose-scaly with split margin; stipe, 4.5~6 cm, equal, hollow, fibrillose, dry, whitish above the superior ring zone, light brown below; crowded gills, adnexed, dark black at maturity. Pileipellis typically cellular with the gill edge appearing white and beaded. Blackish brown basidiospores that discolor in concentrated sulfuric acid. Spores elliptical, warted, 9~11 x 6~8 microm, with prominent snout-like germpores. Cheilocystidia abundant, 57~68 x 19~25 microm, and narrowly elongated clavate, often clustered in threes or fours. Pleurocystidia rarely present, 45~47.5 x 12~13 microm, and clavate to utriform. This trait distinguishes our sample as L. velutina from other Psathyrella spp. of the Coprinaceae, which have smooth spores. This taxon was clarified by the observation that Psathyrella spores fade in concentrated sulfuric acid. A molecular phylogenetic study revealed that our specimen was Lacrymria velutipes, which is closely related to Lacrymaria lacrymabunda. Moreover, those two species are clearly distinguishable from other Psathyrella species, which agreed with the morphologically distinctive traits described above. We believe that this is the first report of this taxon, which has not been described in Korea.

Identification of Diachea leucopodia on Strawberry from Greenhouse in Korea

We have detected the slime mold, Diachea leucopodia (GNU06-10) in a strawberry greenhouse located in Sancheong-gun, Gyeongnam. Typical fruiting bodies had developed gregariously on the strawberry leaves, petioles, and plant debris on ground soil habitat, and also surprisingly on plastic pipes and a vinyl covering. Field samples were examined via stereomicroscopy, light microscopy, and SEM for the determination of morphological characteristics. Dark-brown to black spores formed gregariously within the stipitate cylindrical sporangium, and were covered by an iridescent peridium, which may be intact at maturity, or may have disintegrated. The upper portion of the peridium generally breaks up to expose the spores, whereas the lower portion was usually persistent. The results of energy dispersive X-ray spectrometer (EDS) analysis showed that lime was present in the stalk and columella but absent from the spores, capillitium, and peridium. The above characteristics confirm its taxonomic position in the genus Diachea. However, this genus is intermediate in character between the Physarales and Stemonitales of the Myxogastromycetidae. Hence, this genus had been classified as a member of the Stemonitales until the mid-1970's, on the basis of its iridescent peridium and noncalcareous capillitial system, similar to Comatricha of the Stemonitaceae. By way of contrast, emphasis on morphological characteristics, most notably the calcareous stalk and typical columella, places Diachea within the order Physarales. The presence of a phaneroplasmodium during the trophic stage and lime deposition in its sporophores, as was confirmed in this work, supported the inclusion of Diachea in the Physarales, and the noncalcareous capillitial system verified its identification as a member of the Didymiaceae. Further characteristics of the species D. leucopodia include the following: phaneroplasmodium, spore globose 7.5 microm in diameter, very minutely roughened; sporangia 500 microm x 1mm, more or less cylindrical, gregarious, stalked 1.2mm; stalk and columella white.

Studies on the Effect of Vinyl Mulching on Pleurotus Cultivation: Bunch Formation on Pleurotus sajor-caju (III)

Perforated vinyl mulching technique was performed on Pleurotus sajor-caju beds to assess fruitbody formation. Individual fruitbody of P. sajor-caju was transformed into bunch type on vinyl mulching bed. It was effective to grow the mushroom without waterlogging and abortion of small pins on the beds as well as hygienical bed management. A bunch showed 79 fruitbodies and 225 g of weight. Available site for fruiting was reduced up to 20% in comparison of 100% for conventional bed. The color of fruitbody turned on brownish white from treated vinyl mulching bed.

Effect of speech therapy on alveolar sound after lingual frenulotomy in tongue-tie patients / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Many research papers showed that the most common articulation disorder in tongue-tie children was the error of alveolar sound. We performed speech therapy on alveolar sound after lingual frenulotomy to prove that the speech therapy after lingual frenulotomy improves the articulation of alveolar sound and that this effect is maintained. MATERIALS AND METHODS: We collected 28 children who never had speech therapy but could afford to have the speech therapy. The accuracy rate of consonant sound was calculated using a picture consonant test 2 weeks following the lingual frenulotomy. The speech therapy, called the phonetic placement and paired-stimulus technique, was performed 18 times for 3 months. This therapy decreased incidence rate of error. RESULTS: The incidence rate of alveolar sound error, which was calculated before speech therapy, was about 65%. And the incidence rate of alveolar sound error after speech therapy was 5-10%, which proved the effect of speech therapy. We divided alveolar sounds into target sounds and non-target sounds. And we performed speech therapy only on target sounds so the appearance rate of alveolar sound error decreased and the incidence rate of non-target sounds also decreased. We performed speech therapy after 2 weeks and the effect was maintained. CONCLUSION: The incidence rate of alveolar sound error decreased by using speech therapy and the effect was maintained. So the speech therapy must be performed after lingual frenulotomy.

Effect of Sodium Hypochlorite for Controlling Bacterial Blotch on Pleurotus ostreatus

Sodium hypochlorite alkaline was tested against Pseudomonas tolaasii causing bacterial blotch on cultivated oyster mushroom (Pleurotus ostreatus). The minimum inhibitory concentration of sodium hypochlorite against P. tolaasii contained active chlorine (AC) at 1.4 mg/l on plate assay. The highest cultivation yield was obtained from the treatment of AC 5.7 mg/l. Treatment of sodium hypochlorite at the rate of higher than AC 11.4 mg/l resulted in reduced yields at the harvest. However, the population of total bacteria on the bed surface treated with AC 5.7 mg/l of sodium hypochlorite was maintained to some extent. Inhibitory concentration against total bacteria on the bed surface was over AC 22.8 mg/l. Mushroom mycelium was damaged and its growth strongly inhibited at the concentration of AC 200 mg/l. Mushroom caps showed yellowish symptom by chemical injury by treatments of AC 74.1 mg/l or higher. Sporocarps infected by P. tolaasii were irrevocable at any concentration of sodium hypochlorite. Routine watering with AC 5.7 mg/l from mushroom initiation to the end of picking resulted in reduced bacterial blotch incidence of 40% and 86% at two mushroom farms. The treatment resulted in higher quality mushroom production compared to that conventionally watered with tap water alone.

A Case of CHARGE Syndrome / 대한이비인후과학회지

CHARGE syndroine, first described by Pagon, was named for its six dominant clinical features. They are coloboma of the eye, heart defects, atresia of the choanae, retarded growth and development including CNS anomalies, genital hypoplasia and/or urinary anomalies, and ear anormalies and/or hearing loss, We report a case of CHARGE syndrome with choanal atresia and ear anomalies in the otorhinolaryngologic field, with a review of the previously reported literature concerning CHARGE syndrome.

Surgical Effect of Medpor in the Reconstruction of Orbital Wall Fracture

The records of the patients with the orbital wall fracture were reviewed from January 1985 through December 1994 in the Department of Ophthalmology, Yeungnam University Medical Center. Eighteen patients were repaired orbital wall fracture using Medpor and 44 patients were repaired orbital wall fracture using silicone plate. Surgical effect of Medpor was evaluated and compared with that of silicone plate in the reconstruction of orbital wall fracture. Postoperative improvement of diplopia, extraocular muscle movement, enophthalmos and complications were evaluated after the follow-up period of 6 months. The incidence of the orbital fracture was more common in men than in women(p0.05). the improvement of enophthalmos were 1.2mm in case of Medpor and 1.1mm in silicone plate, but the difference between the two materials was insignificant(p>0.05). Six cases (33%) of the Medpor group and the nine cases (20%) of the silicone plate group caused the infraorbital hypoesthesia, but there was no significant diference between the two groups(p>0.05). Prolapse of the implant was noted in one case of silicone plate implantation, but it was not found in any case of Medpor implantation yet. Postoperative complications such as retrobulbar hematoma, optic nerve injury and lower eyelid retraction were not noted in the two procedures. In this study, Medpor revealed similar surgical effect as silicone palte in reconstruction of orbital wall fracture. However, Medpor has a remarkable characteristic of fibrovascular ingrowth keeping from prolapse or dislocation. Therefore it could be the most ideal implant for the repair of the orbital wall fracture at present.

Comparison Study between the Results of Mini-Microflap Surgery and Microdissection Surgery / 대한이비인후과학회지

Since the anatomy of vocal fold and physiology of phonation are introduced, many surgical techniques for vocal fold disorders has advanced. Microflap and mini-microflap surgery were designed to protect the bare surface of the operated site. However, many new techniques have been based on common sense because of the absence of an accessible animal model with a vocal ligament. Therefore, authors studied to evaluate the differences of the results between mini-microflap surgery and microdissection surgery. Thirty-four mini-microflap surgeries(17 bilateral) and twenty-eight microdissection surgeries(14 bilateral) were performed on 31 patients. Patients' subjective evaluation and videostrobolaryngoscopic examination revealed that mini-microflap surgery was better than microdissection surgery. A mini-microflap surgery, as does limited mucosal resection, was more efficacious in the treatment of vocal nodules than microdissection surgery.

Serum Immunoglobulins in Childrens with Recurrent Sinusitis / 대한이비인후과학회지

BACKGROUND: Sinusitis is a common and frequently recurrent illness in children. Respiratory allergy has been recognized as a major factor that predisposes children to recurrent and chronic sinusitis. Another important causative factors of recurrent sinusitis in children is immunodeficiency diseases. Among them, humoral immunodeficient disease especially is associated with recurrent sinusitis. Most common immune defect in recurrent sinusitis is immunoglobulin deficiency. OBJECTIVES: The aim of this study is to obtain a quantitative data of serum immunoglobulins in children with recurrent sinusitis, to investigate a relationship between recurrent sinusitis and immunoglobulin deficiency. MATERIALS AND METHODS: 30 childrens were selected who had been diagnosed as recurrent sinusitis at Nowon Eulji hospital in 1996. The serum immunoglobulins were evaluated by Latex agglutination immunoassay and ELISA. RESULTS: The serum IgG was within normal limits and IgA deficiency appeared in 1 patient but serum IgM appeared higher than normal value over 3 years patients. The serum IgG subclass deficiency appeared in 3 patients for IgG(1), 7 patients for IgG(2), 14 patients for IgG(3), 10 patients for IgG(4). The combined serum IgG subclass deficiency appeared in 4 patients for IgG(2) and IgG(3), 1 patient for IgG(2) and IgG(4), 4 patient for IgG(3) and IgG(4), 1 patient for IgG(1) and IgG(2) and IgG(3). CONCLUSION: Immunoglobulin deficiency is approved to be closely associated with recurrent sinusitis in children.

Corneal Topographic Evaluation of Centration of Excimer Laser Myopic Photorefractive Keratectomy

In order to get a good visual outcome following excimer laser photorefractive keratectomy(PRK) the centration of ablation is very important. In this study, the ablation center of excimer laser PRK using VISX 20/20 B(R) with VisionKeyTM(VISX, Inc. Santa Clara, CA) was evaluated by using corneal topography. Corneal topography was performed before operation and one month after operation to 190 eyes of 123 patients. The location and distance of center of ablation from center of the entrance pupil were calculated by using EyeSys Corneal Analysis System(R) with pupil-finding software(EyeSys Laboratories, Inc. Houston, TX). Supero-nasal displacement of ablation center occurred in 84 eyes(44%) after excimer laser PRK. The mean distance was 0.33 +/- 0.21mm(range: 0.02~1.36mm). The distance less than 0.5mm was found in 157 eyes(83%) and that between 0.5mm and 0.75mm was found in 26 eyes(14%). The decentering amount following excimer laser PRK was not depending on laterality(OD vs. OS), sex, age, amount of attempted correction(6D down vs. 6D up), type of software(ver 3.20 vs. 4.01) or type of procedure(PRK vs. PARK). In contrast, the second eye receiving excimer laser PRK showed a better centration of ablation than the first eye. And the centration results improved with surgeon's experience in the most recent 50 eyes(0.28 +/- 0.16mm). However, there was no relation between amount of decentration and visual improvement. In this study, it was found that decentering of ablation center less than 0.75mm did not influence the visual outcome in excimer laser PRK.

Prevalence of Central Islands in Corneal Topographic Analysis after Excimer Laser Photo refractive Keratectomy for Myopia

We analyzed the central islands in corneal topography after excimer laser photo refractive keratectomy(VISX Twenty/Twenty B(R) with VisionKey(TM) VISX, Inc. Santa Clara, CA, USA) to evaluate the prevalence of central island and the factors associated with their occurrence, prospectively. Corneal topographic analysis using Corneal Analysis System(EyeSys Technologies, Inc. Houston, TX, USA) was performed in 94 eyes of 77 patients at the postoperative 1-, 3- and 6-months after excimer laser PRK. Central islands were seen in 27%(25/92), 10%(8/79) and 8%(5/64) of patients at postoperative 1-, 3- and 6-months, respectively. There was statistically significant decrease in the occurrence of central island between postoperative 1 and 3 months follow-up groups(p0.05). We found that there was no demonstrable effect of central islands on the decrease of best spectacle corrected visual acuity at postoperative 1 month(p>0.05). Occurrence was statistically significantly decreased in the patients treated with the version 4.01 of central island removal software(1 month: p<0.01, 3 months: p<0.05). We suggest that the version 4.01 software of VISX 20/20B(R) was very effective to reduce the prevalence of central island after excimer laser PRK. In conclusion, if the software would be adjusted to increase the number of pulses centrally, this might reduce the prevalence of central island. New strategies and algorithms might be developed to reduce the prevalence of central islands.