Validation of fetus aneuploidy in 221 Korean clinical samples using noninvasive chromosome examination: Clinical laboratory improvement amendments-certified noninvasive prenatal test

PURPOSE: We developed and validated a fetal trisomy detection method for use as a noninvasive prenatal test (NIPT) including a Clinical Laboratory Improvement Amendments (CLIA)-certified bioinformatics pipeline on a cloud-based computing system using both Illumina and Life Technology sequencing platforms for 221 Korean clinical samples. We determined the necessary proportions of the fetal fraction in the cell-free DNA (cfDNA) sample for NIPT of trisomies 13, 18, and 21 through a limit of quantification (LOQ) test. MATERIALS AND METHODS: Next-generation sequencing libraries from 221 clinical samples and three positive controls were generated using Illumina and Life Technology chemistries. Sequencing results were uploaded to a cloud and mapped on the human reference genome (GRCh37/hg19) using bioinformatics tools. Based on Z-scores calculated by normalization of the mapped read counts, final aneuploidy reports were automatically generated for fetal aneuploidy determination. RESULTS: We identified in total 29 aneuploid samples, and additional analytical methods performed to confirm the results showed that one of these was a false-positive. The LOQ test showed that the proportion of fetal fraction in the cfDNA sample would affect the interpretation of the aneuploidy results. CONCLUSION: Noninvasive chromosome examination (NICE), a CLIA-certified NIPT with a cloud-based bioinformatics platform, showed unambiguous success in fetus aneuploidy detection.

Hope, Depression and Suicidal Ideation in Chronic Mental Illness Patients / 정신간호학회지

PURPOSE: The purpose of this study was to identify the relationship between hope, depression and suicidal ideation in patient with chronic mental illness patients. METHODS: The subjects were 188 patient with chronic mental illness in a Community Mental Health Center. The data were collected from July 27 2009 to August 21, 2009. Data were analyzed using descriptive statistics, pearson correlation coefficients, and stepwise multiple regression. RESULTS: There was found significant and negative correlation among hope and suicidal ideation; significant and positive correlation was found between depression and suicidal ideation; a significant and negative correlation among hope, depression and suicidal ideation. Depression was a significant predictor of suicidal ideation. CONCLUSION: Hope, depression and suicidal ideation in chronic mental illness patients, and significant correlations between their relationships. Based on the outcomes of this study, it is necessary to design an intervention program that emphasizes increasing the hope and decreasing depress in order to decrease suicidal ideation.

Effects of Assertive Training on Interpersonal Relations, Social Behavior, and Psychiatric Symptoms in Patients with a Mental Disorder / 간호학회지

PURPOSE: This study was designed to examine the effects of an assertive training program on interpersonal relations, and psychiatric symptoms in patients with a mental disorder. METHOD: The study employed a quasi experimental design. The subjects included44 patients with a mental disorder, 20 in the experimental group, and 24 in the control group. Data was collected using structured questionnaires over a 3 month period. RESULTS: There were greater significant increases in scores of interpersonal relations and content of communication in the experimental group than the control group. Also, there was a greater significant decrease in the score of psychiatric symptoms in the experimental group than the control group. CONCLUSION: Assertive training has an effect on increasing content of communication and decreasing psychiatric symptoms.