1.
Annals of Clinical Microbiology
;
: 20-23, 2016.
Article
in English
| WPRIM
| ID: wpr-151566
ABSTRACT
In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained.