ABSTRACT
Objective:Based on TGF-β1/Smad3 signal pathway, study the effect of Yiqi-Yangyin-Huoxue-Tongluo Recipe in diabetic rat model. Methods:Forty male SD rats were randomly divided into blank group ( n=10) and diabetic model group ( n=30). Diabetic rats were fed with high sugar and high-fat diet combined with low-dose intraperitoneal injection of STZ. The successful rats were randomly divided into model group ( n=10), traditional Chinese medicine (TCM) group ( n=9) and western medicine group ( n=9). The western medicine group was given trimetazidine hydrochloride tablets 4.16 mg/kg, the TCM group was given Yiqi-Yangyin-Huoxue-Tongluo Recipe 19.1 mg/kg, and the blank group and model group were given the same volume of normal saline, once a day for 20 weeks. The level of fasting blood glucose (FBG) in rats were detected by blood glucose meter; Alkaline hydrolysis was used to detect serum hydroxyproline (Hyp); Enzyme-linked immunosorbent assay (ELISA) to detect serum TGF-β1; Masson staining to observe myocardial fibrosis; Western blotting was used to detect TGF-β1, Smad3, collagen Ⅰ (Col-Ⅰ) and collagen Ⅲ (Col-Ⅲ) protein expression. The mRNA expression of TGF-β1 and Smad3 were detected by real-time quantitative PCR. Results:Compared with the model group, the FBG (19.41 ± 3.65 mmol/L vs. 27.48 ± 2.36 mmol/L), serum Hyp (25.76 ± 1.73 μg/ml vs. 31.71 ± 0.33 μg/ml), TGF-β1 (28.41 ± 0.05 pg/ml vs. 30.82 ± 0.84 pg/ml) of TCM group were significantly reduced ( P<0.05), and myocardial interstitial collagen fibers decreased. The expression of TGF-β1 (0.81 ± 0.02 vs. 0.96 ± 0.08), Smad3 (0.36 ± 0.05 vs. 0.57 ± 0.06), Col-Ⅰ (2.36 ± 0.45 vs. 3.53 ± 0.58), Col-Ⅲ (1.92 ± 0.31 vs. 2.75 ± 0.08) protein in the myocardial tissue of TCM group significantly decreased ( P<0.05), the mRNA expression of TGF-β1 (3.55 ± 0.82 vs. 6.67 ± 1.33) and Smad3 (1.62 ± 0.35 vs. 2.70 ± 0.85) in myocardial tissue significantly decreased ( P<0.05). Conclusions:Yiqi-Yangyin-Huoxue-Tongluo Recipe can effectively treat myocardial fibrosis, and its mechanism may be related to the regulation of TGF-β1/Smad3 signal pathway.