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OBJECTIVE:To establish a method for simultaneous determination of 5 constituents in Phyllanthus emblica,and to conduct principal component analysis and cluster analysis of contents of 5 polyphenolic constituents in P. emblica from different producing areas of Guangxi. METHODS:HPLC method was adopted to determine the contents of gallic acid,epigallocatechin, corilagin,terminalia biphenyl acid and ellagic acid in P. emblica. The determination was performed on Agilent Eclipse XDB column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid aqueous solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 220 nm,and column temperature was 25 ℃. The sample size was 20 μL. The results of content determination was processed by principal component analysis and cluster analysis. RESULTS:The linear range of gallic acid, gallocatechin, corilagin, chebulagic acid and ellagic acid were 1.994-31.90, 0.1598-2.556, 4.533-45.33, 14.75-88.49, 2.956-47.30 μg/mL(r≥0.9998),respectively. The limits of detection were 0.0256,0.0271,0.0529,0.1867,0.1331 μg/mL;the limits of quantitation were 0.0851,0.0893,0.1706,0.6152,0.4419 μg/mL,respectively. RSDs of precision,stability(24 h) and reproducibility tests were all<2.0％(n=6 or n=7). The average recoveries were 96.19％-101.49％(RSD=0.98％-1.41％, n=6). 12 batches of samples had 3 main components. They could be divided into 2 categories. P. emblica from Rong county of Yulin city in Guangxi Zhuang Autonomous Region had highest comprehensive principal component score and best quality. CONCLUSIONS:Established method is simple,accurate and reliable,and can be used for the simultaneous determination of gallic acid,gallocatechin,corilagin,chebulagic acid and ellagic acid in P. emblica. The quality of P. emblica from different producing areas in Guangxi is different.
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Objective To optimize the pretreatment conditions in the detection of selenium from hugan tablets. Methods The main influential factors of pretreatment conditions included concentration of hydrochloric acid,time in water bath and concentration of potassium ferricyanide solution.The extraction effect was evaluated with the content of selenium.Pretreatment conditions were optimized by central composite design-response surface methodology. Results The best pretreatment conditions was 6 mol·L-1hydrochloric acid,30 minutes of bathing in water bath,20%potassium ferricyanide solution. Conclusion The central composite design-response surface methodology is highly predictive,reasonable and feasible.
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<p><b>OBJECTIVE</b>To study the correlation of 1-deoxynojirimycin (DNJ) between Taxilli Herba parasitized in mulberry and its host-plants.</p><p><b>METHOD</b>The contents of DNJ of Taxilli Herba parasitized in mulberry and non-mulberry were determined by RP-HPLC. DNJ was extracted with 0.05 mol x L(-1) HCl, and then detected by fluorescence detector after derivatized with FMOC-Cl at pH 8.0 with borate buffer. The separation was performed on an Agilent C18 (4.6 mm x 250 mm, 5 microm) column with a mobile phase of acetonitrile-0.1% aqueous acetic acid (51: 49) at a flow rate of 1.0 mL x min(-1). The wavelength of fluorescence detector was operated at lambda(EX) = 254 nm and lambda(EM) = 322 nm.</p><p><b>RESULT</b>The linear range of DNJ was 3.72-37.2 mg x L(-1) (r = 0.999 9). The average recovery was 96.42%. The contents of DNJ in mulberry and Taxilli Herba parasitized in mulberry were 1.39-10.16 mg x g(-1) and 0.46-2.72 mg x g(-1), respectively. However, the contents of DNJ could not be detected in Taxilli Herba parasitized in non mulberry and its host-plants.</p><p><b>CONCLUSION</b>As the characteristic constituent of mulberry, DNJ was accumulated in Taxilli Herba This method can be applied to the quality control of Taxilli Herba from mulberry.</p>