Preparation and evaluation of the protective efficacyof pseudomonas aeruginosa irradiated vaccine in chickens

A total of 75 P. aeruginosa isolates were secured out of 280 samples from diseased chickens suffering from different respiratory manifestations. The isolates were characterized and serotyped. The most prevalent serotypes were O3 and O4. An inactivated bivalent P. aeruginosa vaccine was prepared from these serotypes. The inactivation was achieved through irradiation with Cobalt 60. The quality control analysis of the prepared irradiated vaccine indicated that it was safe and its use was not associated with any clinical distress. Application of ELISA for evaluation of humoral immune response developed against P. aeruginosa in the vaccinated chicken groups showed significant increase in antibody titers, which was influenced by the route of immunization. The protection rates post challenge reached 100%, 96% and 90% among the intramuscularly, subcutaneously and orally vaccinated chicken groups, respectively. Also, vaccination of egg laying hens with the prepared vaccine stimulated formation and concentration of P. aeruginosa. specific IgY in the egg yolk

Evaluation of sensitivity and specificity of bovine Gamma-Interferon In-Vitro Assay for Diagnosis of tuberculosis in buffaloes

The sensitivity and specificity of the Bovine Gamma Interferon [IFN-lambda] in-vitro assay [BOVI-GAM] in the diagnosis of bovine tuberculousis in buffaloes as compared to the single intradermal cervical tuberculin test [SICT] and cultural isolation of Mycobacterium bovis were investigated. Thirty buffaloes from a dairy farm, in which tuberculin positive animals had been previously diagnosed, were tested. In the SICT test examined buffaloes 36.7% were positive and 23.3% were doubtful as compared with 53.3% positive and 16.7% doubtful results using the IFN- lambda assay. Two animals were positive to avian tuberculin in the IFN- lambda assay. The prevalence rate reached 60% and 70% using the SICT test and the IFN- lambda assay respectively. The sensitivity of the SICT and the IFN- lambda assay as compared with the cultural isolation of M. bovis were 75% and 95%, respectively, while the specificity were 70% and 80%, respectively. M. bovis was isolated from five buffaloes that were negative to SICT and one buffalo that was negative to the IFN- lambda assay. In conclusion, the present study demonstrated that the IFN- lambda assay is a practical, more sensitive and specific in-vitro diagnostic test than the conventional tuberculin test for detection of bovine tuberculosis in buffaloes