ABSTRACT
Field experiments were conducted at the South farm in Karunya Institute of Technology and Sciences, Division of Agronomy, Coimbatore, during the Rabi season of 2022. The experimental site is situated in the western agro-climatic zone of Tamil Nadu, at a latitude of 10°56'N and longitude of 76°44'E, with an elevation of 474 meters above mean sea level. The soil composition of the experimental field was identified as clay loam, moderately drained, and exhibited low availability of nitrogen (202 kg/ha), high availability of phosphorus (12.3 kg/ha), medium availability of potassium (415 kg/ha), and organic carbon content of 0.51%. The experiment followed a randomized block design (RBD) with 10 treatment groups, each replicated three times viz.,T1 - Control, T2 - Vermicompost 100% (on N Equivalent basis)+ Cowdung slurry, T3 -Vermicompost100% (on N Equivalent basis)+TNAU Biomineralizer,T4 -Vermicompost100% (on N Equivalent basis) + (Effective microorganism),T5 - Vermicompost 100% (on N Equivalent basis) + Pleurotus sp + Urea, T6- Vermicompost (50%) +RDF (50%) + Cowdung slurry, T7- Vermicompost (50%) +RDF (50%) + TNAU Biomineralizer, T8 -Vermicompost (50%) + RDF (50%) + EM (Effective Microorganism),T9 -Vermicompost (50%) + RDF (50%) + Pleurotus sp ,T10 - RDF alone. The objective of the study was to investigate the impact of vermicompost and other foliar applications on the growth, physiological characteristics, and yield parameters of an irrigated Black gram (VBN8 variety). The promotion of yield can be attributed to the increased vegetative growth and the balanced carbon-to-nitrogen (C: N) ratio, which likely stimulated the synthesis of carbohydrates. This, in turn, facilitated the overall improvement in seed yield. The application of RDF played a significant role in enhancing growth, leading to various positive changes in yield-related characteristics. Ultimately, the increased seed yield observed can be largely attributed to the improved plant growth and subsequent positive effects on yield-related attributes resulting from the RDF application. Thus, From this Experiment it was Concluded that the RDF (T10) Shows a highest range in growth, physiological and yield parameters of irrigated Blackgram due.
ABSTRACT
A field experiment was conducted during the rabi season of 2022 in the farm of Karunya Institute of Technology and Sciences, Coimbatore to study the effect on organic nutrient management on yield and economic returns of baby corn which was grown in silty clay loam soil. Among all the treatment 12.5 t FYM + groundnut cake equivalent to 60 kg fertilizer N provided much better outcomes in terms of yield attributing character and cob yield followed by vermi-compost equivalent to N in 12.5 t FYM + groundnut cake equivalent to 60 Kg fertilizer N. B:C ratio was found higher in 60-30-30 kg NPK through fertilizer alone.
ABSTRACT
Methicillin Resistant Staphylococcus aureus (MRSA) strains have emerged as one of the most important nosocomial pathogens. The MRSA can cause a wide range of diseases, which is associated with its production to large number of extracellular toxins and other virulence factors. The diseases are toxic shock syndrome, scalded skin syndrome and food poisoning. Hospital-acquired MRSA (HA-MRSA) in persons who have had frequent or recent contact with hospitals or healthcare facilities within the previous year, has recently undergone an invasive medical procedure, or is immunocompromised. Mostly HA-MRSA are transmitted most frequently through direct skin-to-skin contact or contact with shared items or surfaces that have come into contact with someone else’s colonized or infected skin. Panton Valentine Leukocidin (PVL) is a biocomponent toxin has been shown to induce lysis of host defence cells. The absence of the PVL gene confirms the MRSA as HA-MRSA. Slime layer plays a remarkable role in bacterial colonization of exterior surfaces by adhesion and production of slime factor plays an important role in antibiotic resistance. Beta lactamases render bacteria resistant to beta-lactam antibiotics by hydrolyzing the beta lactam ring of penicillin’s and cephalosporins.There is a linear correlation between beta-lactamase activity and the level of resistance of bacteria to penicillins. The phage groups II and III were present in hospital acquired MRSA which colonizes on the normal skin and enter the body through cut/wound or by fracture and cause osteomyelitis and bacterial arthritis. Bacteriophage typing of MRSA strains is an epidemiological marker and is a successful method in strain characterization.
ABSTRACT
Development of multi drug resistant organism has been high due to improper use of antibiotics. That made the necessity to develop new drug molecules. In this study an effort was made to find a new alternative. A wild type microorganism was isolated from soil and was identified as Bacillus and confirmed as Bacillus subtilis species by 16S r RNA sequencing. The strain was identified to have the ability to produce bacteriocin by stab overlay assay. Bacteriocin was produced in nutrient broth and that was extracted by organic solvent extraction using chloroform and further purification was carried out by HPLC and the molecular weight of the bacteriocin was analysed by SDSPAGE. Antimicrobial activity was analysed on four strains Pseudomonas sp, Staphylococcus sp, Klebsiella sp and Proteus sp. and was found to be sensitive towards the analyzed strains.