Comparative study on the microleakage of three root canal sealers

The aim of this study was to examine and compare the apical sealing ability of AH26, AH Plus and AH Plus Jet using the fluid filtration model. In this experimental study, 70 single-rooted teeth were dissected from the cement-enamel junction. Canals were prepared with ProTaper rotary system and hand K-files and irrigated with 5.25% NaOCl and 17% EDTA. Ten teeth were assigned to the control group and divided into 5 positive and 5 negative controls. Remaining specimens were divided into 3 groups of 20 samples each and filled with gutta percha by lateral condensation technique. Each sample group was filled with either AH26, AH Plus or AH Plus Jet. Microleakage was assessed on the 2[nd] and 30[th] day by the amount of air bubble movement within the capillary glass tube connected to the root. One-way ANOVA test was used for analysis. AH Plus Jet had the least microleakage value and AH Plus presented the highest rate; however, the differences were not statistically significant. Under the conditions of this study, all three studied sealers provided satisfactory seal within the two time intervals. AH Plus Jet demonstrated slightly lower microleakage values; therefore, its application can be recommended in endodontic therapy

in vitro comparative study on the antimicrobial effects of bioglass 45S5 vs. calcium hydroxide on Enterococcus faecalis

An ideal intracanal medicament should be able to eliminate any remaining intracanal microorganism. The aim of this study was to compare the antimicrobial effects of Bioglass 45S5 with calcium hydroxide on Enterococcus [E] faecalis in-vitro. Direct exposure test [DET] was used to evaluate the antimicrobial effect of Bioglass 45S5, calcium hydroxide and normal saline [control group] on 80 paper cones contaminated with E. faecalis suspension. All samples were aseptically transferred into BHI culture medium to quantify microbial concentration in periods of 1, 24, 48 and 72 hours. Turbidity of the culture medium was measured via optical density [OPD] method with a spectrophotometer [wavelength=540nm]. Results were then analysed statistically using student t-test. Mean difference of optical density between Bioglass 45S5 and calcium hydroxide appeared insignificant within 1 hour of the test period [P>0.05]; however calcium hydroxide showed significantly greater antimicrobial properties after 24 hours [P<0.05]. Antimicrobial effect of both materials displayed significant increases with time. Although both Bioglass 45S5 and calcium hydroxide exhibited antimicrobial effects against E. faecalis, neither attained complete eradication of bacteria. However, calcium hydroxide seemed to have superior disinfecting effect