Isolation and biochemical identification of pathogenic burkholderia cepacia front human sources and related reactivity to different antibiotics

The aim of the present work is to isolate and identify Burkholderia species from different chest hospitalized patients .throat swap and sputum samples were considered. Reactivity of isolates to different antibiotics was considered using both Double Disc Synergy Test and Combined ESBL Test. Data revealed that 21/200 [10.5%] isolated from cystic flbrosis patients; samples were positive as Burkholderia isolation was more prevalent from sputum samples [55%] than throat swapping [45%]. Among the 21 bacterial isolates; 2/21[9.5%] children, 8/21[38.09%] females and 11/21[52.38%] males. The No of isolate was location related; El Sadr hospital [41%], Abbassia Chest hospital [24%], Al-Mattaria teaching hospital [21%] and health institute hospital [14%]. Also, isolation was gender related as male was more susceptible more than the other genders for infection by Burkholderia cepacia recording the highest value [52%] followed by female [38%] and children [10%]. Combined infection was recorded. Burkholderia cepacia was sensitive to Amlkacin, Gentamicin, Kanamycin, Neomycin, Ceftazidime, Ciprofloxacin and Amoxicillin/clavulanic acid, while it were resistant to; Paromomycin, Spectinomycin, Fosfomycin, Cefixime, Levofloxacin, Ofloxacin and Cefazolin. The most active antibiotic was Amoxicillin/clavulanic acid with mean diameter of inhibition zone 38.50 mm

Phylogenetic identification ofburkholderia cepacia isolates from human chest infection from different hospitals

Phylogenetic analysis of Burkholderia cepacia was achieved based on the variation of p-Lactamases production and antibacterial reactivity to different antibiotics. Sub inhibitory concentrations of augmentin down-regulates the production and/or release of exoprotein and this effect increase with increasing of the tested concentration. ESBLs was detected using double disc synergy test and combined ESBL test. Effect of reactivity of B. cepacia spp was influenced by antibiotic where Amoxicillin/ clavulanic acid showed the highest inhibitory effect and in turn clear zone dimension. Also, TEM-1 and SHV-1 genes electrophoretic pattern detected by both PFGE and RAPD. Different isolates showed a similarity to standard B. cepacia ranging from 94-95 %. Data recorded revealed the p-Lactamases genes showed a variable protein bands pattern revealing reactivity to antibiotics