ABSTRACT
TNFa has been associated with both, tumor survival and apoptosis. This cytokine is also involved in promoting cell migration during wound healing and tumorigenesis. SW756 is a HPV18-positive cervical carcinoma cell line, which has been used to study different mechanisms of cervical cancer progression. An in vitro assay of scratch wound healing onto monolayers of SW756 cells was used to assess the effect of TNFa on cell migration into a wound space. It was found that SW756 cells have the ability to migrate, but not proliferate in response to scratch wounding in a serum-free medium supplemented with TNFa. RT-PCR analysis showed that SW756 cells express TNFa mRNA when incubated in medium with and without serum. Wound closure and migration rate of SW756 cells were significantly increased in the presence of serum-free media supplemented with TNFa (10 ng/mL) as compared to serum-free media, and media supplemented with either anti-TNFa antibody or both TNFa and anti-TNFa antibody (p<0.05). The results showed a stimulatory effect of TNFa on the migration of SW756 cervical carcinoma cells, suggesting a novel and important role for TNFa in cervical cancer progression.
Subject(s)
Female , Humans , Carcinoma/microbiology , Cell Movement/drug effects , /genetics , Tumor Necrosis Factor-alpha/pharmacology , Uterine Cervical Neoplasms/microbiology , Cell Line, Tumor , Culture Media, Serum-Free , Carcinoma/genetics , Carcinoma/pathology , Cell Proliferation/drug effects , /isolation & purification , Image Processing, Computer-Assisted , Kinetics , Microscopy, Video , Reverse Transcriptase Polymerase Chain Reaction , RNA, Messenger/metabolism , Recombinant Proteins/drug effects , Uterine Cervical Neoplasms/genetics , Wound Healing/drug effectsABSTRACT
De un total de 112 pacientes estudiados durante cuatro anos (1976-1980) se seleccionaron 8 con diagnostico de neumonia intersticial linfoide. Se describen los hallazgos de los estudios realizados, haciendo mencion especial de la correlacion observada entre evolucion clinica con tratamiento y pruebas funcionales respiratorias
Subject(s)
Adult , Humans , Male , Female , Pulmonary Fibrosis , Microscopy, Electron , Prednisone , Respiratory Function TestsABSTRACT
Veintidos pacientes hospitalizados con neumonia aguda, bronquiectasias, absceso pulmonar o empiema, fueron tratados con cefoperazone, 2 g en 250 ml de solucion glucosada al 5%, aplicada por una hora y administrada cada 12 horas. El tratamiento se continuo por 10 dias en la mayoria de los pacientes. El farmaco mostro tener actividad contra bacterias grampositivas y gramnegativas, especialmente estas ultimas, entre las cuales fue en particular efectivo contra Proteus indol positivo, Enterobacter, Serratia marcescens y Pseudomonas aeruginosa. Tambien es activo contra anaerobios incluyendo Bacteroides fragilia. En este estudio los organismos mas sensitivos al cefoperazone fueron K. pneumoniae y Streptococcus viridans. Se obtuvo curacion clinica en 10 pacientes; 11 mejoraron y 1 murio por insuficiencia cardiaca. Tambien hubo curacion bacteriologica en 16 pacientes; en 5 persistio la bacteria y en 1 no se pudo hacer la evaluacion. Cefoperazone ha mostrado ser eficaz en el tratamiento de infecciones respiratorias, especialmente las debidas a bacterias gramnegativas resistentes a otros antibioticos