Applied research into the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs

Introduction: The need for gama \u2013 globulin, especially gama - globulin - anti \u2013 HBs, is huge in Vietnam. A number of patients cannot to afford use them due to the high price as they are imported. Meanwhile, Vietnam has high quality input sources for producing gama \u2013 globulin. \r\n', u'Objectives: To study the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs. \r\n', u'Subjects and method: 168 samples of human plasma from voluntary blood donors, which had been screened with for transfusion transmittable infections (TTLs), were chosen as plasma with rich of anti - HBs. The plasma with anti - HBsAg was precipitated with ethanol 25%, pH 6,9 to gain gama - globulin with rich anti - HBs, which was dried by Dutch Ly - 3 - TTE machine. Activation of anti - HBs gama - globulin was identified by a standard method of the degree of antibody specific for anti - HBsAg kit of BIORAD, \r\n', u'Results: The purity of the gama - globulin achieved was 93%, which was almost equal with the results of some foreign researchers (7.8), the activation of anti - HBs was 1:128 dilution degree. The productivity of gama - globulin gaining from 1 litter plasma was 6.0 gram. This new issue was first demonstrated in Vietnam. \r\n', u'Conclusion: We can domestically produce anti-Bs gama - globulin with high degree of activeness (1:128) from human plasma by the precipitating method with ethanol, pH and low temperature. \r\n', u'

Result of production of standard Albumin from human plasma

Background: Human albumin was produced and used in many countries. Cohn's technique had been used to precipitate albumin from human plasma. This technique was easy and cheap and the quality of the product was good. In Vietnam, human albumin had to import, but the prices was very expensive. Vietnam was having good plasma in large quantity and high quality. That\u2019s why research on production plasma albumin was essential.\r\n", u"Objectives: This study aimed at using Cohn's technique improved by Drohan and Van - Aken to produce standard albumin from human plasma. Subjects and method: Human plasma detected VIII-factor was used for present study. Plasma \ufffd?albumin was precipitated by ethanol at low temperature and pH. The collected albumins have been liophilizated and storage at 40C. The quality and quantity of Albumin was evaluated by quantitative analysis and protein \ufffd?electrophoresis. Results: The 418g of albumin powder was produced from 16 liters of plasma detected F \ufffd?VIII. The quality of this albumin come up to standard (>95%) and quantity of albumin collected from one liter of this plasma was 26g. Conclusion: In the Vietnamese condition, the technique of Cohn can be used to produce standard albumin for treatment.\r\n", u'

Results of medical arthropod surveys in the natural conserve zone- Can Gio mangrove forests, Ho Chi Minh city

Background: Can Gio mangrove forest is bio-diversified area. There is few studies on medical arthropod in Can Gio mangrove forest until now. Objective: To study bio-diversified feature and identify species with disease transmitting role. Subject and Method: In 2007, medical arthropod surveys were conducted at 3 sites located in the natural conserve zone- Can Gio mangrove forest. A cross-sectional study was used. Results and Conclusions: A total of samples of 6178 individuals of medical arthropod belonging to 65 species have been collected, including 3 species of flea (Siphonaptera), 1 species of ticks (Ixodidae), 6 species of chiggers (Trombiculidae), 6 species of mites (Gamasoidea), 25 species of flies (Muscoidea) and 24 species of mosquitoes (Culicidae). They belong to 37 genus, 17 families, 3 orders (Siphonaptera, Acarina and Diptera), and 2 classes (Insecta and Arachnida). 20 species having epidemiological role were found in Can Gio mangrove forest. The rate of Anopheles epiroticus biting human at night indoor, outdoor and daytime indoor is rather high, especially at the end of dry season at centre of the forest.

Result of investigation on medical arthropod in some place along Ho Chi Minh road in Cao Bang province

Background: In the period 2006-2010, a investigation on medical arthropod has been conducted\r\n', u'Objective: 1) To determine species composition and distribution of medical arthropod. 2) To find species transmitted disease \r\n', u'Subject and method: The cross sectional, descriptive and analytic investigation was conducted in 3 communes along the Ho Chi Minh road in Cao Bang province. \r\n', u'Results and conclusion: : A total of 3.437 of samples of medical arthropod belonging to 96 species have been collected including: three species of flea (Siphonaptera), two species of tick (Ixodoidea), four species of chigges (Trombiculidae), ten species of mites (Gamasoidea), twenty four species of flies (Muscoidea), and fifty-three species of mosquito (Culicidae). They belong to 39 genuses, 17 families, 3 orders: (Siphonaptera, Acarina and Diptera); two classes (Insecta and Arachnida). \r\n', u'Some species having epidemiological role such as Aedes albopictus, Culex tritaeniorhyncus, Cx. quinquefasciatus, Cx. vishnui ... were predominant at all study sites. Anopheles minimus, the main malaria vector, was mainly collected in cattle- sheds in Truong Ha and Bach Dang communes. \r\n', u'