ABSTRACT
To Study Therapeutic Effect of Carnitine on Atorvastatin-induced Mechanical Myotoxicity of Gastrocnemius Muscles of Rats. Observational study. This study was conducted at Department of Physiology, University of Karachi, from 21[st] January 2012 to 30[th] December 2013. Present study showed that effect of statin on mechanical properties of gastrocnemius muscle of rats and use of carnitine as prevention of statin induced myopathies. Animals were injected statin for 6 weeks in one group and carnitine to another group along with statin to study the possible therapeutic effect. After treatment period, animals were decapitated and gastrocnemius muscles were isolated. Twitch and tetanus of muscles were recorded in each group. Our results showed that treatment of statin reduced the body weight of animals and increased the resting length [106%] of isolated gastrocnemius muscles. We also observed that force of contraction of both twitch and tetanus in statin treated group were significantly reduced [P>0.0001]. This negative effect of statin on twitch and tetanus parameters of muscle was partially decreased by an additive treatment of carnitine. Thus, carnitine plays a vital role in improving muscle contractile ability caused by statins. Our study demonstrated the potential preventive measure of atorvastatin-induced myopathy using carnitine and its impacts on mechanical function of muscles
ABSTRACT
Attempt was made to study the factors that affect maximum yield of nonprotein nitrogen from various skeletal muscles. The results showed that sonification was the most effective method out of the various extraction procedures used for the extraction of NPN, while the use of tungstic acid as protein precipitant was most suitable. The pH studies showed that pH 2 gave the optimal yield of NPN. Results of the coloured sample dilutions showed exponential relationship between optical densities and the concentrations of NPN used. The results are discussed in terms of the factors affecting maximum yield of NPN along with the significance of the term half optical density dilution
Subject(s)
Nitrogen , Ultrasonics , Hydrogen-Ion Concentration , Animals, LaboratoryABSTRACT
Effect of chronic denervation and temperature was studied on the force velocity relationship of rat diaphragm muscle. The muscles were denervated for 15 - 20 days and force of contraction and velocity of shortening were calculated according to Hill [1938] using isotonic contraction data. The results showed that the force velocity relationships were not altered at 35°C to 25°C. However, a change in this parameter was observed in the denervated muscles at 15°C. It is suggested that the velocity of shortening is independent of changes in the series elasticity of the denervated muscle at higher temperatures