Genetically Independent Tetranucleotide to Hexanucleotide Core Motif SSR Markers for Identifying Lentinula edodes Cultivars

For the purpose of protecting the rights of Lentinula edodes breeders, we developed a new simple sequence repeat (SSR) marker set consisting only of genetically independent tetranucleotide or longer core motifs. Using available genome sequences for five L. edodes strains, we designed primers for 13 SSR markers that amplified polymorphic sequences in 20 L. edodes cultivars. We evaluated the independence of every possible marker pair based on genotype data. Consequently, eight genetically independent markers were selected. The polymorphic information content values of the markers ranged from 0.269 to 0.764, with an average of 0.409. The markers could distinguish among 20 L. edodes cultivars and produced highly repeatable and reproducible results. The markers developed in this study will enable the precise identification of L. edodes cultivars, and may be useful for protecting breeders' rights.

Inflection to Pharmacovigilance of PreAVOID / 薬剤疫学

The Japanese Society of Hospital Pharmacists (JSHP) started to collect the pharmaceutical care reports from the hospital pharmacists by a name of “PreAVOID” from 1999. The report number of PreAVOID increased year by year and reached about 50,000 totals in 2006. PreAVOID reports are classified in 2. One is the prevention of the adverse reaction and the drug interaction, the other is the avoidance of progress to the adverse reaction. In the latter, the role of the adverse reaction monitoring is included. Therefore PreAVOID is able to apply in Pharmacovigilance. PreAVOID is reported to JSHP by Fax or E-mail or the homepage of JSHP and it is evaluated and classified and then are accumulated in the database. The accumulated PreAVOID reports are utilized for creation of the pharmaceutical care information, study promotion about the medical economy. Therefore PreAVOID takes an important role as a nucleus of the pharmaceutical care management system by the pharmacists.<br> We are convinced that PreAVOID will be able to contribute to achievement of real purpose of Pharmacovigilance that is the effective and safety drug therapy.