Expression of the mRNA of Growth Factor Receptors and Neurotrophic Factors in Porcine Vesical Submucosa and Acellular Matrix / 대한비뇨기과학회지

PURPOSE: Several naturally-derived biopolymers have been introduced as implantable biomaterial for tissue reconstruction, and some of them are already being applied in various clinical fields. They serve as backbones for the regeneration of damaged tissue. It is well known that growth factors play an important role in this process and some of the naturally- derived biopolymers were known to contain several kinds of growth factors, but there are few reports about any growth factors in the various biopolymers. We tried to confirm the existence of growth factor receptors and neurogenic factors through the expression of the mRNA from the biopolymers. MATERIALS AND METHODS: Bladders were harvested from adult pigs. Vesical submucosa was obtained with using mechanical decellulization technique. Acellular matrices were made from pig bladder with using a cell lysis technique, and they were examined by performing scanning electron microscopy. Expression of the mRNA of vascular endothelial growth factor (VEGF) receptor, fibroblast growth factor (FGF)-1 receptor, neuregulin and brain-derived neurotrophic factor (BDNF) in the acellular matrix and the submucosa of porcine bladder were examined for via reverse transcription- polymerase chain reaction (RT-PCR). RESULTS: The ultrastructure of the acelluar matrix shows collagen fibers with many pores. The mRNA's of VEGF receptor, FGF-1 receptor and neuregulin were expressed in the porcine vesical acellular matrix, whereas BDNF was not expressed. On the other hand, none of the mRNA's examined was expressed in the porcine vesical submucosa. CONCLUSIONS: This study's results shows that several growth factors are detected in the acellular matrix of the porcine bladder. These growth factors may play an important role for the growth of tissue when it is implanted in vivo. It is also assumed that there are more growth factors in naturally-derived biopolymers, and it would be useful to investigate the undefined components of naturally-derived biopolymers for developing new and advanced polymers.

Papillary Urothelial Tumor of Bladder in 5-year-old Girl / 대한비뇨기과학회지

The occurrence of bladder tumors in the first 2 decades of life are rare neoplasias. Transitional cell tumors of the bladder are extremely a rare clinical disease entity. Here, a case of a papillary urothelial tumor in a 5-year-old girl is reported, with a brief review of the literature. Histologically, the tumor was a solitary and papillary urothelial tumor of low malignant potential (by WHO and the ISUP).

Regeneration of Renal Tissue Using Cell Transplantation in a Renal Failure Rat Model / 대한이식학회지

PURPOSE: Dialysis and renal transplantation, the current therapies for end-stage renal disease (ESRD), have limitations including severe complications, donor organ shortage, and graft failure. The present study investigated the possibility of using a tissue engineering technique for renal tissue reconstruction as a new method to replace the current treatments for ESRD. We restored renal structure in vivo by transplanting isolated renal cells in renal failure animal models. METHODS: Renal failure was surgically induced by 5/6 nephrectomy using silk tie method in Sprague-Dawley (SD) rats. Renal failure was confirmed by measuring the concentrations of blood urea nitrogen (BUN) and creatinine from blood samples. Renal cells were freshly isolated from newborn SD rat kidneys and implanted into renal failure- induced kidneys with fibrin gel matrix for 4 weeks. Retrieved specimens were examined by histological analyses. RESULTS: Renal failure-induced rats exhibited higher concentrations of BUN and creatinine compared to those of normal rats. Four weeks after cell transplantation, histological examination showed the reconstitution of vascular tufts of glomerular structures. CONCLUSION: Renal failure rat models were successfully created by 5/6 nephrectomy. This study showed a possibility of restoring the renal structures by transplanting renal cells with fibrin gel matrix in renal failure rat models. Further studies, such as investigation on renal function recovery by cell transplantation, are necessary to determine the clinical utility of this method for partial or full replacement of renal structure and function in the treatment of ESRD.