Comparing the effects of fast and slow expansion on nasal cavity and maxilla structure / 华西口腔医学杂志

OBJECTIVE@#This study aims to compare the effects of fast and slow expansion on nasal cavity structure.@*METHODS@#A total of 40 patients were selected and randomly divided into two groups. Cone-beam computer tomography (CBCT) was obtained before and after surgery and used for comparing the changes in nasal structure before and after treatment.@*RESULTS@#Fast expansion had resulted in greater changes in the basilar and nasal bone arch extension structures than slow expansion. No significant difference at maxillary width and nasal parenchyma.@*CONCLUSIONS@#Rapid expansion therapy has more beneficial effects on nasal function.

Characteristics of dental arch and basal bone in permanent dentition Angle Class Ⅱ malocclusion based on three-dimensional digital models / 中国组织工程研究

BACKGROUND:An orthodontist can gain all comprehensive data about the tooth and jaw based on a model measurement.With the development of CAD/CAM,a three-dimensional (3D) digital model shows more accurate information than a traditional model.OBJECTIVE:To explore the characteristics of dental arch and basal bone in permanent dentition Angle Class Ⅱ malocclusion based on 3D digital models.METHODS:Thirty cases of normal occlusionin permanent dentition were selected as control group,and 30 cases of Angel Ⅱ1 and 30 cases of Angel Ⅱ2 malocclusion were enrolled,respectively.Three kinds of 3D digital models were established using a 3shape R700 scanner and measured with Orthoanalyzer 2013.The data of individual normal occlusion and Angel Ⅱ1 and Angel Ⅱ2 data were analyzed statistically with SPSS 19.0 software respectively.Intergroup comparison was analyzed by the least significant difference test method.RESULTS AND CONCLUSION:Maxillary and mandibular dental arch length and maxillary dental arch width of front section (3-3) and middle section (4-4)/maxillary basal bone length and mandibular basal bone width of middle section (4-4):Ⅱ2 ＞ normal occlusion ＞ Ⅱ1 (P ＜ 0.05).Mandibular dental arch front width of section (3-3)/maxillary and mandibular basal bone length:normal occlusion ＞ Ⅱ1＞ Ⅱ2 (P ＜ 0.05).Maxillary dental arch width of behind section (6-6)/maxillary basal bone width of front section (3-3) and middle section (4-4)/mandibular basal bone width of front section (3-3):normal occlusion ＞ Ⅱ2 ＞ Ⅱ1 (P ＜ 0.05).The angle of tooth long axis and plane:there was significant difference in the Angel Ⅱ1,Ⅱ2 and normal occlusion (P ＜ 0.05).Maxillary posterior-teeth area (coronary):Ⅱ1 ＞ Ⅱ2 ＞ normal occlusion (P ＜ 0.05).Mandibular posterior-teeth area (coronary):Ⅱ2 ＞ normal occlusion ＞ Ⅱ1 (P＜ 0.05).The dental arch of Angel Ⅱ1 was narrow and long in shpape,and the dental arch of Angel Ⅱ2 was wide and short in shape.Sagittal discordant existed in the anterior of Angel Ⅱ1,such as lip-side incline of maxilla and mandible.Coronal discordant existed in the median and posteior of Angel Ⅱ1,such as palatine-side incline of the maxillary dental arch,and buccal-side incline of the mandible.Sagittal discordant existed in the anterior of Angel Ⅱ2,e.g.lingual-side incline of the maxilla and mandible.No discordant existed in the posterior section of Angel Ⅱ2.

Graded ethanol precipitation method on physicochemical properties and antioxidant activities of polysaccharides extracted from Astragalus Radix / 中国中药杂志

Astragalus polysaccharide has been widely used in food and medicinal industry owing to its health-promoting properties. In order to characterize better the relationship among molecular weight, structure-activity and activities, a simple method was used different concentration of ethanol including 30% (PW30), 50% (PW50), 70% (PW70), 75% (PW75), 80% (PW80) and 90% (PW90) to precipitate Astragalus polysaccharides into different molecular weight. As a result, PW90 showed smooth surface and the strongest antioxidant activity among these six fractions (P < 0.05). In conclusion, graded ethanol precipitation was a simple method to separate Astragalus polysaccharides into different molecular weight with different antioxidant activity fractions.

Regularity of drugs compatibility of anti-hepatoma traditional Chinese medicine ancient prescriptions and risk evaluation of anti-hepatoma new drug research and development / 中国中药杂志

Traditional Chinese ancient prescriptions have been used for treatment of liver cancer for a long history and the scientific and rational compatibility is a great wealth for modern research and development (R&D) of new drugs. The research and development of new drugs are often accompanied with a large investment, a long cycle and a high risk, especially for the anti-tumor drugs R&D which are facing more risks and lower successful rate. In this research, the regularity of compatibility of drugs was analyzed from 124 anti-hepatoma ancient prescriptions by computer program. The results can offer help to the R&D of anti-hepatoma new drugs and reduce the risk of drug screening. In addition, we surveyed 22 companies in this field from six provinces such as Beijing, Shanghai, Tianjin and so on and obtained 240 risk assessment questionaires. Then we used qualitative analysis method to interpret the greatest impacts for the risks in the process of R&D, production and sales of anti-hepatoma new drugs. The study provides a basis for anti-liver cancer drugs R&D researchers, who can take effective measures to reduce the R&D risks and improve successful rate.

Dynamic monitoring risk of anti-hepatoma new drug development / 中国中药杂志

Risk monitoring of new Chinese patent anti-hepatoma drugs is tracking recognized risks and residual risks, identifying emerging risk and ensure the implementation of the plan, estimating the process of reducing effectiveness. The paper is mainly through understanding the status of Chinese patent anti-hepatoma drugs, the content, characteristic and analysis method of dynamic risk monitoring, and then select the risk control indicators, collect risk information. Finally, puts forward the thought of anti-hepatoma drugs listed evaluation in our country, and try to establish the model of dynamic risk management of anti-hepatoma drugs.

Relationship between abnormal swallowing and mouth breathing / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the relationship between abnormal swallowing and mouth breathing.</p><p><b>METHODS</b>Thirty-eight patients with abnormal swallowing and 38 patients with normal swallowing were selected. All patients presented with no airway constriction. The age range of the patients was 11-14 years old. The number of patients with mouth breathing was calculated. Statistical analysis (χ(2) test) was performed.</p><p><b>RESULTS</b>The number of patients with mouth breathing in the abnormal swallowing group (17, 45%) was significantly higher than that in the normal swallowing group (5, 13%) (χ(2) = 9.212, P = 0.002).</p><p><b>CONCLUSIONS</b>Abnormal swallowing was related to mouth breathing.</p>

Expression of Osterix mRNA and protein levels in cultured human periodontal ligament cells after mechanical stimulation / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To examine the expression of Osterix (Osx) mRNA and protein after application of mechanical force on human periodontal ligament cells (HPDLCs), and to investigate the role of Osx in orthodontic alveolar bone remodeling.</p><p><b>METHODS</b>HPDLCs were isolated and cultured in vitro with explant method. Approximately 2.5 x 10(5) cells were seeded onto six-well cell culture plates and then were exposed to centrifugal force for 1, 2, 4, 6, 8 or 12 h at 631 r x min(-1). The expression of Osx mRNA and protein was measured by reverse transcription-polymerase chain raction (RT-PCR) and Western blot respectively. Immunofluorescence assay was used to detect the expression and subcellular At the initial time point, Osx mRNA had a weak exlocalization of Osx protein by green fluorescence.</p><p><b>RESULTS</b>pression and protein was not detected. Under the mechanical stimulation, both mRNA and protein levels of Osx were upregulated in a time-dependent manner. Furthermore, Osx protein was translocated gradually from the cytosol into the cell nuclei.</p><p><b>CONCLUSION</b>The expression and activation of Osx were enhanced by mechanical stress in HPDLCs, which indicates that Osx may play an important role in HPDLCs osteogenic differentiation and periodontal tissue remodeling induced by mechanical stress.</p>

Detection and significance of phosphatidylinositol 3-kinase in adipose tissue of polycystic ovary syndrome patients with insulin resistance / 中华妇产科杂志

Objective To investigate the expression of phosphatidylinositol 3-kinase(PI-3K)in adipose tissue of polycystic ovary syndrome patients(PCOS),and explore molecular mechanisms of insulin resistance(IR)in PCOS.Methods Samples from patients with PCOS with IR(n=19),PCOS without IR (n=10)and controls(n=15)were collected.Serum fasting insulin(FIN)and fasting plasma glucose (FPG)were measure& Insulin resistance index was calculated using homeostasis model assessment (HOMA)to analyze the relationship between these markers and IR.Western blot technique was used to detect the PI-3K p85 subunit.Gene expression of PI-3K p85 subunit was detected by reverse transcription polymerase chain reaction(RT-PCR)method.Kinase activity was detected by immunoprecipitation,thin- layer chromatography and gamma scintillation counting.Results(1)The levels of FIN[(25.2?3.8) mU/L]and HOMA-IR(1.6?0.3)in PCOS with IR were significantly higher than those in PCOS without IR[(13.4 +3.8)mU/L,0.9?0.3]and controls[(9.5 +2.6)mU/L,0.5?0.3;all P＜0.05).(2) There was no significant difference in the protein(0.65?0.10)and gene expression(0.92?0.12)of PI-3 K p85 subunit in PCOS with IR compared with PCOS without IR(0.72?0.10,1.01?0.10)and control groups(0.73?0.14,1.00?0.12;P＞0.05).(3)PI-3K activity in PCOS with IR(81%)and PCOS without IR(89%)was significantly decreased(P＜0.01,P＜0.05)and negatively correlated with HOMA- IR(r=-0.69,P＜0.01;r=-0.62,P＜0.05).Conclusions No significant difference in the protein and gene expression of PI-3K p85 subunit in PCOS with IR is found.The decreased PI-3K activity may lead to IR of PCOS.