ABSTRACT
<p><b>OBJECTIVE</b>To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.</p><p><b>METHODS</b>Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.</p><p><b>RESULTS</b>In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.</p><p><b>CONCLUSION</b>There is a correlation between La protein and HBV mRNA and the expression of HBV protein.</p>