ABSTRACT
AIM:To investigate the effects of sinapine,an effective monomer of Chinese medicine,on hydro-gen peroxide(H2O2)-induced adipogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).METHODS:The undifferentiated rat BMSCs were identified and screened by flow cytometry.The adipogenic differentiation of BMSCs was induced by H2O2,and the toxicity of sinapine on BMSCs was tested by CCK-8 assay.After the modeling method and the concentration range of sinapine were determined,the lipid droplets in the cells were detected by Oil Red O semi-quanti-tative assay,and the optimal drug concentration was selected.Finally,Oil Red O assay was observed 24 h after drug inter-vention,and the expression of adipogenic differentiation-related proteins,adipocyte protein 2(aP2),peroxisome prolifera-tor-activated receptor γ(PPARγ)and glucose transporter 4(Glut4),at mRNA and protein levels in the BMSCs was deter-mined by qPCR and Western blot.RESULTS:Treatment with H2O2at 200 μmol/L for 1 h induced BMSCs to differentiate into adipocytes.Below the concentration of 40 μmol/L,sinapine had no toxicity to BMSCs.The best inhibitory concentra-tion of sinapine on adipogenic differentiation was at 15 μmol/L.The number of lipid droplets in sinapine(15 μmol/L) group was significantly lower than that in model group.In sinapine group,the expression of aP2,PPARγand Glut4 at mR-NA and protein levels was lower than that in model group(P<0.01).CONCLUSION: Sinapine inhibits H2O2-induced adipogenic differentiation of rat BMSCs.The mechanism may be related to the PPARγ/AMPK signaling pathway.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of HSP70 and P53 in malignant tumor tissues of patients with TCM Heat-Syndrome (HS) or with non-Heat-Syndrome (NHS).</p><p><b>METHODS</b>S-P immunohistochemical method was used to detect the gene expression, and ELISA and RT-PCR were adopted to determine the content of HSP70 and P53 mRNA expression in different malignant tumor tissues.</p><p><b>RESULTS</b>(1) Positive ratio of HSP70 and P53 in the colorectal cancer of HS was significantly higher than that in colorectal cancer of NHS respectively, and the total positive ratio of the two genes in all tumors of HS was higher than those of NHS (P<0.05); (2) HSP70 expression content in colorectal, nasopharyngeal and lung cancers of HS was higher than in those of NHS respectively, and the total HSP70 expression content in tumors of HS was higher than in those of NHS (P<0.01); (3) HSP70 mRNA expression content in colorectal and lung cancers of HS was higher than in those of NHS respectively, and the total HSP70 mRNA expression content in tumors of HS was higher than in those of NHS (P<0.01).</p><p><b>CONCLUSION</b>HSP70 and P53 positive ratio and HSP70 expression content in patients of malignant tumor with HS was higher than in those with NHS.</p>