ABSTRACT
<p><b>OBJECTIVE</b>To identify dihydroflavonol glycoside isomers in Smilax glabra.</p><p><b>METHOD</b>The sample was analyzed by HPLC-MS in combination with HPLC-1H-NMR.</p><p><b>RESULT</b>Four dihydroflavonol glycoside isomers were identified as astilbin, neoastilbin, isoastilbin, neoisoastilbin.</p><p><b>CONCLUSION</b>The method is simple and rapid for the identification of dihydroflavonol glycosides in S. glabra.</p>
Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Chemistry , Flavonols , Chemistry , Glycosides , Chemistry , Isomerism , Magnetic Resonance Spectroscopy , Mass Spectrometry , Smilax , ChemistryABSTRACT
<p><b>AIM</b>To establish a comprehensive HPLC analytical method of Huanglianjiedu decoction.</p><p><b>METHODS</b>This study was performed by HPLC-UV/MS to identify the chemical constituents of the whole and individual herbs of the "Huanglianjiedu decoction". Zorbax Extend C18 (150 mm x 4. 6 mm ID, 5 microm) column was used; the mobile phase was composed of acetonitrile (A) and water (B, with 0.5% acetic acid) with gradient elution; the flow rate was 1.0 mL x min(-1) and the column temperature was setup at 25 degrees C. The detection wavelength was 254 nm.</p><p><b>RESULTS</b>The chromatogram of Huanglianjiedu decoction showed 21 main peaks. Peaks 1, 2, 5 and 18 were from Gardenia jasminoides Ellis, Peaks 8, 13, 14, 15, 16, 17, 19 and 21 from Scutellaria baicalensis Georgi. While 10 from Coptis chinensis Franch and 20 from Phellodendron amurense Rupr., Peaks 3, 4, 6, 9, 11 and 12 came from them together. Peak 7 presented in the chromatograms of the herbs except Gardenia jasminoides Ellis. By comparison of the retention time, the on-line UV spectra and MS spectra, 11 peaks were identified as 5 (geniposide), 9 (jatrorrhizine), 10 (coptisine), 11 (palmatine), 12 (berberine), 13 (baicalin), 15 (oroxin A), 17 (wogonoside), 19 (baicalein), 20 (obaculactone), 21 (wogonin), then eight of them were quantified by HPLC-UV.</p><p><b>CONCLUSION</b>The method could represent the characteristics of Huanglianjiedu decoction, and it could be used to evaluate the quality and quantity of Huanglianjiedu decoction. It distinguished between Coptis chinensis Franch and Phellodendron amurense Rupr. by HPLC for the first time.</p>
Subject(s)
Berberine , Berberine Alkaloids , Chromatography, High Pressure Liquid , Methods , Coptis , Chemistry , Drugs, Chinese Herbal , Chemistry , Gardenia , Chemistry , Mass Spectrometry , Methods , Phellodendron , Chemistry , Plants, Medicinal , Chemistry , Quality Control , Scutellaria baicalensis , Chemistry , Spectrophotometry, Ultraviolet , MethodsABSTRACT
<p><b>AIM</b>To identify the main metabolites of aconitine in the urine of rabbits.</p><p><b>METHODS</b>After oral administration of aconitine (5 mg.kg-1), the urine of male rabbits was collected and extracted by solid phase extraction and analyzed by liquid chromatography-ion trap mass spectrometry.</p><p><b>RESULTS</b>Aconitine and 4 metabolites were found in the rabbit urine. Their protonated molecular ions at m/z 632, m/z 604, m/z 590, m/z 500 and multistage fragment ions with neutral loss of 60 u, 32 u, 28 u and 18 u were monitored. Their relative concentration were M1 > Aconitine > M4 > M2 > M3.</p><p><b>CONCLUSION</b>The metabolites M1-M4 were deduced as 16-O-demethylaconitine, benzoylaconine, 16-O-demethylbenzoylaconine and aconine, respectively.</p>