ABSTRACT
Curcumin, a polyphenolic compound from the plant Curcuma longa L., has shown a wide-spectrum of anti-inflammatory and antitumor activities. Despite the promising biological effects of curcumin, its poor solubility has restricted its use in the management of human ailments. To improve its water-solubility, curcumin succinate prodrugs were designed and synthesized and their aqueous solubility, stability, metabolism in rats and anti-inflammatory activity were evaluated (experiments had been approved by the ethics committee and carried out in accordance with the relevant guidelines and regulations; rats were provided by Beijing courtyard experimental animal center of Academy of Military Medical Sciences). The results showed that curcumin succinate prodrugs are very soluble in water and more stable than curcumin in water and in phosphate buffer solution. They released curcumin rapidly and quantitatively after intravenous administration. In phlogogen-induced paw edema in rats, curcumin succinate prodrugs showed anti-inflammatory activity as potent as dexamethasone.
ABSTRACT
OBJECTIVE: To investigate the impurity profile of cloxacillin sodium for injection and study the source of major impurities. METHODS: The impurities in samples from seven manufacturers were profiled by an optimized reversed-phase HPLC method and a newly established normal-phase HPLC method. The major impurities were prepared by semi preparation technology and identified by LC-QTOF-MS, 1H-NMR and 13C-NMR. RESULTS: Two new impurities relating with the salt-forming reaction and the purification process respectively, and four other new impurities were all successfully identified in this study. CONCLUSION: The study is suitable for profiling the impurities in cloxacillin sodium for injection and is quite useful for enhancing the quality control.