ABSTRACT
Objective:To explore the effect and mechanism of pilose antler different components on the bone tissue of ovariectomized osteoporosis model rats and ascertain the material basis of pilose antler. Method:fifty-six SD rats were divided randomly into seven groups:normal group,model group,Xianling Gubao group(468 mg·kg-1),Bujiale group(80 mg·kg-1),polysaccharide group(50 mg·kg-1),polypeptides group(175 mg·kg-1),polysaccharide and polypeptide mixture group(50 mg·kg-1+175 mg·kg-1). Osteoporosis mode was established through ovary resection of female rats,meanwhile,the rats were given different components of pilose antler for consecutively 12 weeks. Subsequently, using absorptiometry to measure the rats' bone mass density. The activities of bone alkaline phosphatase(BALP),osteocalcin (OT),bone morphogenetic protein2(BMP-2),Smad1,Smad5,Runt-related transcription factor 2 (RUNX2) were detected by enzyme-linked immuno sorbent assay (ELISA). The expression of BMP-2,Smad1,Smad5,Runx2 protein was examined by Western blot and Real-time polymerase chain reaction (Real-time PCR). Morphological assay for bone tissue were detected by htoxylin eosin(HE) staining. Result:After 12 weeks, Compared with the normal group, the osteoporosis model group showed significantly decrease in bone mineral density(PPPConclusion:Pilose antler different components has therapeutic effect on ovariectomized osteoporosis model rats.The mechanism may be related to up-regulat the expression of BMP-2/Smad1,Smad5/Runx2 signal pathways.
ABSTRACT
AIM:To determine the expression patterns of bone morphogenetic protein(BMP)signaling com-ponents in the mammary glands with hyperplasia(MGH)and the activation of this signaling pathway.METHODS: The female C57BL/C mice(8-weeks-old)were randomly divided into control group and hormone group with 15 mice in each group.The mice in control group were treated with PBS,while the mice in hormone group were intragastric administration with estradiol valerate(2.5 mg/kg)for 25 d,followed by progesterone(4 mg/kg)peritoneal injection for 5 d.At the end of treatments,the mammary glands were collected to determine the morphological changes, the mRNA expression of BMP signaling components and the phosphorylation level of Smad 1/5/9.RESULTS:In MGH,a part of BMP signaling pathway molecules were differentially expressed(P<0.05),including BMP ligands BMP2,4,5,6,7,9,13 and 14, BMP re-ceptor BMPR1A, and antagonists Chrdl1 and Twsg, whereas the expression levels of some molecules, such as BMP3, BMP12,BMPR1B,BMPR2,Chrd,Chrdl2 and Noggin, were not altered.The phosphorylation levels of Smad 1/5/9 was up-regulated in MGH(P<0.05).CONCLUSION: BMP signaling pathway is activated in MGH through the abnormal expression of its components.BMP signaling pathway may be a new target for MGH therapy.