Lung transplantation after paraquat poisoning in an adolescent: one case report and literature review / 器官移植

Objective To summarize the effect of the timing of lung transplantation and related treatment measures on clinical prognosis of patients with paraquat poisoning. Methods Clinical data of a patient with paraquat poisoning undergoing bilateral lung transplantation were retrospectively analyzed. Clinical manifestations, auxiliary examination, diagnosis and treatment of this patient were summarized and analyzed. Results A 17-year-old adolescent was admitted to hospital due to nausea, vomiting, cough and systemic fatigue after oral intake of 20-30 mL of 25% paraquat. After symptomatic support treatment, the oxygen saturation was not improved, and pulmonary fibrosis continued to progress. Therefore, sequential bilateral lung transplantation was performed under extracorporeal membrane oxygenation (ECMO). After postoperative rehabilitation and active prevention and treatment for postoperative complications, the patient was discharged at postoperative 50 d. Conclusions The timing of lung transplantation after paraquat poisoning may be selected when the liver and kidney function start to recover. Active and targeted prevention of potential pathogen infection in perioperative period and early rehabilitation training contribute to improving clinical prognosis of lung transplant recipients.

Effect of Cinobufacin injection combined with chemotherapy on serum tumor markers and immune function in elderly patients with colorectal cancer / 中国医师进修杂志

Objective:To investigate the short-term effect of Cinobufacin injection combined with chemotherapy on elderly patients with colorectal cancer and its influence on tumor markers and immune function.Methods:Sixty-two elderly patients with colorectal cancer admitted to Xinchang Branch of Zhejiang Hospital of Traditional Chinese Medicine from January 2015 to December 2017 were randomly divided into observation group (31 cases) and control group (31 cases). Patients in the control group were treated with chemotherapy, while those in the observation group were treated with Cinobufacin injection on the basis of the control group. Two treatment of 21 d was as a cycle, continuous chemotherapy 2-4 cycles. The short-term therapeutic effect, Karnofsky function state score (KPS score), serum tumor markers, immune function and side effects of the two groups were compared before and after treatment.Results:After treatment, the total effective rate of the observation group was 77.42%(24/31), and was higher than that of the control group 48.39%(15/31), there was significant difference ( χ2=5.599, P<0.05). The KPS scores of the observation group was higher than that of the control group [(87.48 ± 6.57) scores vs. (80.20 ± 6.12) scores], there was significant difference ( t=4.514, P<0.05). The levels of serum cancer antigen 19-9(CA19-9), carbohydrate antigen 724(CA724) and carcino-embryonic antigen (CEA) in the observation group were lower than those in the control group [(3.59 ± 1.02) kU/L vs. (6.98 ± 1.37) kU/L, (7.38 ± 1.87) μg/L vs.(9.82 ± 1.42) μg/L, (12.27 ± 2.36) μg/L vs.(16.57 ± 3.24) μg/L], there were significant differences ( t=11.051, 9.042, 10.624, P<0.05). The levels of CD 3+, CD 4+ and CD 4+/CD 8+ in the observation group were higher than those in the control group [(58.97 ± 3.72)% vs. (47.31 ± 2.98)%, (38.85 ± 3.25)% vs. (30.19 ± 2.71)%, 1.59 ± 0.18 vs. 0.89 ± 0.14], there were significant differences ( t=13.620, 11.394, 17.091, P<0.05). The incidence of side effects in the observation group was lower than that in the control group ( P<0.05). Conclusions:Cinobufacin injection combined with chemotherapy can improve the quality of life of elderly patients with colorectal cancer in the near future, reduce the levels of serum CA19-9, CA724 and CEA, improve the immune function of patients, and have fewer side effects.

Modification study of cyanoacrylate medical adhesive / 医疗卫生装备

Objective To perform modification study of cyanoacrylate (CA) medical adhesive and to select the optimal mo-difying material and the best ratio.Methods Carboxylic multi-walled carbon nanotubes (MWCNTs-COOH), multi-walled carbon nanotubes (MWCNTs), hydrophobic nano-silica, nitrile rubber, epoxy resin and polymethyl methacrylate (PMMA) were used to modify 5% triclosan/cyanoacrylate antimicrobial adhesive respectively. The bond strength, toughness and viscosity of the modified adhesive were examined in different concentrations.Results 0.064% MWCNTs-COOH, 6% nano-silica, 4% nitrile rubber and 6.4% epoxy resin all had good effects in strength modification. The bond strength were (14.71±1.48)MPa, (14.03±1.92)MPa, (14.6±1.78)MPa and (14.05±1.46)MPa respectively. 8% nano-silica had the lowest Tg of (1.1±0.24)℃ and the strongest viscosity of (15 536.68±28.4)cP. When the nano-silica concentration was 8%, the compound was very viscous and sticky. When the antimicrobial adhesive modified with 6% nano-silica, the bond strength was (14.03±1.92)MPa, the Tg was (3.6±0.68)℃, and the viscosity was (5 278.87±31.68)cP.Conclusion 6% nano-silica is the best modifying material, and has the optimal effect of modification.

A novel method of defective vascular reconstruction using 2-octyl-cyanoacrylate and homemade prosthetic component / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Currently, adhesive technique is popular in vascular repair but not widely used for defective vessels. This study aimed to determine the feasibility and effectiveness of repairing defective vessels with 2-octyl-cyanoacrylate and a homemade prosthetic component.</p><p><b>METHODS</b>Homemade prosthetic component consisting of expanded polytetrofluoroethylene (ePTFE), terylene film, and homemade soluble hollow stent mixed with adhesive can replace autologous graft and suture in repairing defective vessels, can fix vessels better using the stent without occlusive bleeding. Forty male mongrel dogs were used, 20 for biomechanical tests and 20 for animal experiments. In the biomechanical test, dogs were randomly divided into two groups (n = 10 each), one group repaired on the two sides of the carotid arteries with 2-octyl-cyanoacrylate and homemade component and another group repaired with suture and ePTFE. Of the 40 specimens, 10 were used for adhesive and 10 for suture specimens for tension strength test, whereas the remaining specimens were used for bursting pressure test. In animal experiments, dogs were also divided into adhesive and suture groups (n = 10), only of the left carotid artery. Recording the operational time, bleeding or not. Vessels were tested using color Doppler ultrasound, the inner diameter was measured, and the degree of stenosis at 8 weeks was evaluated digital subtraction angiography (DSA) were also performed. Specimens were then analyzed histologically.</p><p><b>RESULTS</b>In the adhesive and suture groups, the specimens could afford atension strength of (23.80 ± 1.51) N versus (24.60 ± 1.08) N (P > 0.05), the bursting pressure was (52.03 ± 2.43) kPa versus (50.04 ± 3.51) kPa (P > 0.05), and the mean time of anastomosis was (15.20 ± 0.55) minutes versus (25.97 ± 0.58) minutes (P < 0.05). One dog in the adhesive group was bleeding from the suture. One dog from each group presented with thrombosis at 1 week. After measuring using ultrasound, the stenosis degree of all dogs were no more than 30% except the two thromboses. DSA and histological observation showed no obvious difference between the two groups.</p><p><b>CONCLUSION</b>Defective vascular anastomosis with 2-octyl-cyanoacrylate and our homemade prosthetic component is feasible, effective, timesaving, and easy to master.</p>

Experimental study on new self and mutual-aiding occlusive dressing for wound / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Self and mutual-aiding occlusive dressing is a novel method to treat with the wounds in special circumstances. This study aims to prepare a new antimicrobial adhesive for the dressing and evaluate the application effects of the adhesive.</p><p><b>METHODS</b>The main component of the new antimicrobial adhesive was 5% triclosan / cyanoacrylate (CA) antimicrobial adhesive. The adhesive was modified with carboxylic multi-walled carbon nanotubes (MWCNTs-COOH), multi-walled carbon nanotubes (MWCNTs), hydrophobic nano-silica, nitrile rubber, epoxy resin and polymethyl methacrylate (PMMA) respectively. The bond strength, toughness and viscosity of the modified adhesive in different concentrations were examined to select the optimal modifying material and the best ratio to prepare the new antimicrobial adhesive according to the results. After that, the antimicrobial property of the new antimicrobial adhesive was tested by filter paper method. At last, we disposed the injury models in rats using the new antimicrobial adhesive to examine the application effects.</p><p><b>RESULTS</b>In individual tests, the bond strength modification performance of 0.064% MWCNTS-COOH is the best, the bond strength is (14.71 ± 1.48) Mpa. 8% nano-silica shows the best toughness modification performance, the Tg is (1.10 ± 0.24)°C. The viscosity modification performance of 8% nano-silica is the best, the viscosity is (15 536.68 ± 28.4) cP. However, consolidating three test results, 6% nano-silica/antimicrobial adhesive has the balanced bond strength, toughness and viscosity. Its bond strength is (14.03±1.92) Mpa, the Tg is (3.60 ± 0.68)°C, and the viscosity is (5 278.87 ± 31.68) cP. The inhibition zone diameter of 6% nano-silica/antimicrobial adhesive and antimicrobial adhesive group in Day 5 is (28.61 ± 0.91) mm versus (28.24 ± 2.69) mm (P > 0.05). In animal studies, both in blood routine test and pathological section, 6% nano-silica/antimicrobial adhesive group shows lower white blood cells count than gauze bandage group (P < 0.05).</p><p><b>CONCLUSIONS</b>6% nano-silica has the optimal effect of bond strength modification, toughness modification and viscosity modification, and the antimicrobial adhesive modified with it has a good antimicrobial property (resistant staphylococcus aureus).</p>

Study on Chemical Constituents of Citrus mediea var. sarcodactylis from Sichuan Province (Ⅱ) / 中药新药与临床药理

Objective To investigate the chemical constituents of Citrus medica var. sareodactylis from Sichuan province, and to provide evidence for the development and utilization and quality evaluation of the medicinal material.Methods Various chromatographic techniques were used to purify the components of this herb. Compounds were identi-fied by their physical characteristics and spectral feature. Results Seven compounds were isolated from Citrus medica var. sarcodactylis, and they were identified as sibiricol (Ⅵ), 7-methylesculetin (Ⅶ), bergapten (Ⅷ), sigmasteryl acetate(Ⅸ), 5-methoxyfurfural(Ⅹ), limonin(Ⅺ), daucosterol (Ⅻ). Conclusion Compound Ⅵ, Ⅶ and Ⅸ are isolated from plants of Rutaceae for the first time.

Determination of Lasiokaurin in the Stems and Leaves of Rabdosia nervosa by HPLC / 广州中医药大学学报

Objective To develop a HPLC method for the determination of Lasiokaurin in the stems and leaves of Rabdosia nervosa.Methods HPLC was performed on a Kromsil C18 reversed-phase column(250mm?4.2mm,5?m).All of the reference substances and samples were separated with the mobile phase of methanol :water(55:45) under isocratic elution for 30min,flow rate was 1.0ml/min,the detection wavelength was 234nm,and the column temperature was 30℃.Results The content of Lasiokaurin was 0.005mg/g in the stems and 0.372mg/g in the leaves,the content in the stems were almost 1/75 times as much as that in the leaves.The average recovery of Lasiokaurin was 96.21%.Conclusion This developed method,which is simple and with good precision,high sensitivity and selectivity,can be used for quality evaluation of Lasiokaurin in Rabdosia nervosa.

Determination of Oridonin in Rabdosia nervosa by HPLC / 中药新药与临床药理

Objective To develop a HPLC method for the determination of oridonin in the leaves and stems of Rabdosia nervosa.Methods All of reference substances and samples were separated with the mobile phase of methanol:water (45 ∶55) under isocratic elution for 20 min on a Kromasil C18 reversed-phase column(250 mm?4.0 mm,5 ?m),the flow rate was 1.0 mL?min-1,the detection wavelength was 235 nm,and the column temperature was 30 ℃.Results The content of oridonin was 0.047 mg/g in stems and 0.791 mg/g in leaves,the content in stems being about one seventeenth of that in leaves.The average recovery of oridonin was 98.33 %.Conclusion This method is simple,sensitive,accurate and suitable for quantitative determination of oridonin in Rabdosia nervosa.

Determination of Icariin Content in Qianlie Shule Granule by HPLC / 中药新药与临床药理

Objective To determine the icariin content in Qianlie Shule Granules (QSG). Methods After proper pre-treatment,HPLC method was performed on a C18 column. The mobile phase consisted of acetonitrile-water (29 ∶71) and the UV d etective wavelength was set at 270 nm. Results The peak of icariin was separated completely. The calibration curve of icariin was linear within 0.16～1.2 mg?mL -1,Y=0.028 7X-0.009 62 (r=0.999 9). Conclusion This method is accurate and su itable for the determination of icariin content.